Inhibition of Proliferation and Induction of Apoptosis of HT-29 Colorectal Adenocarcinoma Cells in Vitro by Anthocyanins from Lonicera Edulis

Abstract:

Anthocyanins was known for their antioxidant and pharmacological properties used by humans for therapeutic purposes. Here, we study the inhibitory effects of anthocyanins from Lonicera edulis on the proliferation of HT-29 colorectal adenocarcinoma cells and its possible mechanism in vivo by MTT. The different percentages of apoptotic cells present in flow cytometry analysis. The effect on HT29 cells was enhanced with increasing amount of anthocyanins. Typical morphological features of apoptosis were observed by transmission electron microscope. The mechanism of anthocyanins from Lonicera edulis on HT29 cell growth inhibition may be related to the induction of apoptosis.

• Periodical: Advanced Materials Research (Volumes 233-235)
• Pages: 2945-2948
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.233-235.2945
• Online since: May 2011
• Authors: Yi Hong Bao, Wen Xing Li, Zhen Yu Wang
• Keywords: Anthocyanin, Apoptosis, HT-29 Colorectal Adenocarcinoma Cell, Lonicera edulis

References

[1] Ohnishi T., Takahahashi, A: Takeda K.Mutation Research/Environmental/Mutagenesis and Related Subjects.Vol.32(1991),pp.541-547

[2] Sherwin HW, Farrant JM: Plant Growth Reg.Vol.24(1998),pp.203-210

[3] Jin-Ming Konga, Lian-Sai Chiaa, Ngoh-Khang Goha, Tet-Fatt Chiaa, R. Brouillardb: Phytochemistry.Vol.64(2003),pp.923-933

[4] Li-Shu Wang, Gary D. Stone: Cancer letters.Vol.269(2008),pp.281-290

[5] Weiqin Jiang, Zongjian Zhu: Cancer Reseach.Vol.62(2002),pp.5280-5287

[6] Masahiko Imai，Hidetomo Kikuchi，Takanori Denda，et al: Cancer Letters.Vol.276(2009),pp.74-80

[7] Y. Choi, D. Shin1, S. Kim, S. Lee, et al: Poster Session.Vol.7(2008),p.127

[8] Becker H: Agricultural Research.Vol.49(2001),p.22

[9] Konga J M, Chiaa L S, Goha N K, et al: Phytochemistry.Vol.64(2003),pp.923-933

[10] Yun-ching Chang, Hui-pei Huang, Jeng-dong Hsu, et al: Toxicology and Applied Pharmacology.Vol.205(2005),pp.201-212

[11] J. Dai, A. Guote, L. Gates:Food and Chemical Toxicology.Vol.64(2003),pp.923-933

[12] Micnael Netzel, Gabriele Netzel, Dietmar R. Kammerer: Innovative Food Science and Emerging Technologies.Vol.8(2007),pp.365-372

DOI: 10.1016/j.ifset.2007.03.011

[13] Monica Jordheim, Nlls Harald Giske, Øyvind M. Andersen, et al: Biochemical Systematics and Ecology.Vol.35(2007),pp.153-159

DOI: 10.1016/j.bse.2006.09.010

[14] Shih P H,Yen C T,Yen G C: Food Chem Toxicol.Vol.43(2005),pp.1557-1566

[15] Chen P N,Chu S C,Chiou H L, et al.: Cancer Lett.Vol.235(2006),pp.248-259

[16] Meiers S.,kemeny M.,Weyand U: Agric Food Chemical.Vol.49(2001),pp.958-962

[17] Lamy S,Lafleur R,Bedard V: J Cell Biochem.Vol.100(2007),pp.100-111