Astaxanthin Production by Phaffia Rhodozyma Fermentation of Pineapple Peel Substrate

Article Preview

Abstract:

Pineapple peel as main material was fermented with Phaffia rhodozyma to produce astaxanthin. Using the Box-Behnken design, the effects of sugar content, initial pH and nitrogen content were studied with the yield of astaxanthin as response value, which was evaluated to optimize the fermentation conditions of astaxanthin production. The optimal fermentation conditions have been reached by the study: sugar content was 36.3 g/L, the initial pH was at 5.85 and nitrogen content was 8 g/L. By validation test, the astaxanthin yield under the optimal condition, which was basically corresponded to the model prediction, was 97.11 %.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 233-235)

Pages:

2962-2969

Citation:

Online since:

May 2011

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2011 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] Bjerkeng.B., Storebakken, T., Liaaen Jensen, S.1990. Response to carotenoids by rainbow trout in the sea: Resorption and metabolism of dietary astaxanthin and canthaxanthin. Aquaculture 91: 153–162.

DOI: 10.1016/0044-8486(90)90184-o

Google Scholar

[2] Johnson,E. A., Villa, T.Lewis, M.1980. Phaffia rhodozyma as an astaxanthin source in salmonid diets. Aquaculture 20: 123–134.

DOI: 10.1016/0044-8486(80)90041-1

Google Scholar

[3] Sigurgisladottir, S., Parrish, C. C., Ackman, R. G., Lall, S. P. 1994a. Tocopherol deposition in the muscle of atlantic salmon (Salmo salar). J.Food Sci. 59: 256–259.

DOI: 10.1111/j.1365-2621.1994.tb06942.x

Google Scholar

[4] Sigurgisladottir, S., Parrish, C. C., Lall, S. P., Ackman, R. G. 1994b. Effects of natural tocopherols and astaxanthin on atlantic salmon (Salmosalar) fillet quality. Food Res. Int. 27: 23–32.

DOI: 10.1016/0963-9969(94)90174-0

Google Scholar

[5] Johnson, E.A. and Schroeder, W.A. (1995) Microbial carotenoids production. Adv Biochem Eng 53, 119–178.

Google Scholar

[6] Guerin, M.Huntley, M.E. and Olaizola, M. (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotechnol 21, 210–216.

DOI: 10.1016/s0167-7799(03)00078-7

Google Scholar

[7] An GH, Bielich J, Auerbach R, Johnson EA (1991) Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting. Biotechnology 9:70–73

DOI: 10.1038/nbt0191-70

Google Scholar

[8] Olaizola M (2000) Commercial production of astaxanthin from Haematococcus pluvialis using 25,000-liter outdoor photobioreactors. J Appl Phycol 12:499–506

Google Scholar

[9] Andrewes, A.G., Phaff, H.J. and Starr, M.P. (1976) Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting yeast. Phytochemistry 15, 1003–1007.

DOI: 10.1016/s0031-9422(00)84390-3

Google Scholar

[10] Johnson, E. A., An, G.-H. 1991. Astaxanthin from microbial sources. Crit.Rev. Biotechnol. 11: 297–326.

Google Scholar

[11] Haard NF(1988) Astaxanthin formation bythe yeast Phaffia rhodozyma on molasses. Biotechnol Lett 10:609–614

DOI: 10.1007/bf01024710

Google Scholar

[12] Fontana JD, Guimaraes MF, Martins NT, Fontana CA, Baron M (1996) Culture of the astaxanthin ogenic yeast Phaffia rhodozyma in low-cost media. Appl Biochem Biotechnol 57–58:413–422

DOI: 10.1007/978-1-4612-0223-3_38

Google Scholar

[13] Hayman TG, Mannarelli BN, Leathers TD (1995) Production of carotenoids by Phaffia rhodozyma grown on media composed of corn wet-milling co-products. J Ind Microbiol 115:173–183

DOI: 10.1007/bf01569956

Google Scholar

[14] Okagbue RN, Lewis MW (1984) Autolysis of the red yeast Phaffia rhodozyma: a potential tool to facilitate extraction of astaxanthin. Biotechnol Lett 6:247

DOI: 10.1007/bf00140045

Google Scholar

[15] Meyer PS, du Preez JC (1994) Astaxanthin production by Phaffia rhodozyma mutant on grape juice. World J MicrobiolBiotechnol 10:178–183

DOI: 10.1007/bf00360882

Google Scholar

[16] Acheampong E, Martin A (1995) Kinetic studies on the yeast Phaffia rhodozyma. J Basic Microbiol 35:147–155

DOI: 10.1002/jobm.3620350304

Google Scholar

[17] Martin AM, Acheampong E, Patel TR (1993)Production of astaxanthin by Phaffia rhodozyma using peat hydrolysates assubstrate. J Chem Tech Biotechnol 58:223–230

DOI: 10.1002/jctb.280580304

Google Scholar

[18] DU Si-juan,MEI Yan-zhen,HU Yao-hui,et al. Optimization of Culture Conditions for Producing Astaxanthin by Phaffia rhodozyma[J].Food Science,2008,29(8):441-444. (china)

Google Scholar