Simultaneous Resolution of Clenbuterol Hydrochloride Enantiomers and Propranolol Hydrochloride Enantiomers using Thin-Layer Chromatography

Abstract:

Resolution of clenbuterol hydrochloride enantiomers and propranolol hydrochloride enantiomers was achieved by thin-layer chromatography using methyl-β-cyclodextrin impregnated silica gel GF254 plates. The effects of stereoselective auxiliary (acetonitrile/different alcohols) were studied. Resolution of clenbuterol hydrochloride enantiomers could be realized by alcohols such as ethanol, n-butanol, 2-butanol or tert-butanol together with acetonitrile, and propranolol hydrochloride enantiomers could only be resoluted by ethanol or n-butanol together with acetonitrile. The optical conditions of simultaneous resolution were obtained as follows: 15.00g silica gel GF254 plates impregnated with 1.00g methyl-β-cyclodextrin, acetonitrile/n-butanol (V/V = 90:10) mixed solvent as spreading solvent, being resolved under ambient temperature. Under these conditions, R_F value of resolution of clenbuterol hydrochloride enantiomers is 3.47, R_F value of resolution of propranolol hydrochloride enantiomers is 3.35. The two couples of enantiomers were all achieved base line separation.

• Periodical: Advanced Materials Research (Volumes 236-238)
• Pages: 1546-1548
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.236-238.1546
• Online since: May 2011
• Authors: Jin Gang Yu, Du Shu Huang
• Keywords: Clenbuterol Hydrochloride Enantiomers, Methyl-β-Cyclodextrin, Propranolol Hydrochloride Enantiomers, Resolution, Thin-Layer Chromatography

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