With current status of medical staffing and healthcare resources, identifying the issues in the process of treatment with hospital ship receiving and delivering mass casualty is key to more utilize the existing health care resources so as to achieve more effective medical treatment at sea. The method based on Queuing Theory is employed to establish systematic model for the medical treatment process on hospital ships. It has integrated the available healthcare resources into casualty variations and set up a systematic simulation model. Through the study of above process, it can realize visual display of process data and statistic analysis, which allows for obtaining various indicators and rescue process data related to the actual rescue ability on hospital ships and identifying the key bottlenecks during the rescue. The system can flexibly meet the needs of different conditions and can provide basis for the medical resource allocation and process optimization of hospital ship. It also offers new technical means and methods for resolving complicated issues through quantitative research.