Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)₃²⁺

Abstract:

The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridization events in a more rapid, simplistic and cost-effective manner compared to conventional methods. Here, we present an electrochemical DNA biosensor for the specific and quantitative detection of single-stranded DNA (ssDNA). Probe oligonucleotides were immobilized onto gold electrodes by a 5′ end thiol-group linker. Following hybridization with the complementary DNA, the cobalt complex was electrochemically accumulated on the double-stranded DNA layer and the differential pulse voltammogram (DPV) for this electrode gave an electrochemical signal due to the redox reaction of [Co(phen)₃]²⁺/³⁺ that was bound to the double-stranded DNA on the electrode, and it can sense single base-pair mismatche within the sequence of the hybridized double-stranded DNA (dsDNA).

• Periodical: Advanced Materials Research (Volumes 301-303)
• Pages: 213-218
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.301-303.213
• Online since: July 2011
• Authors: Cun Zhou, Bo Wen Cheng, Xiao Ping Fan, Zhao Dai, Guo Zheng, Qing Yin Zhang, Guang Ping Chen
• Keywords: DPV, Electrochemical DNA Biosensor, Electrochemical Indicator, Thiol-Group

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