Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)32+

Abstract:

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The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridization events in a more rapid, simplistic and cost-effective manner compared to conventional methods. Here, we present an electrochemical DNA biosensor for the specific and quantitative detection of single-stranded DNA (ssDNA). Probe oligonucleotides were immobilized onto gold electrodes by a 5′ end thiol-group linker. Following hybridization with the complementary DNA, the cobalt complex was electrochemically accumulated on the double-stranded DNA layer and the differential pulse voltammogram (DPV) for this electrode gave an electrochemical signal due to the redox reaction of [Co(phen)3]2+/3+ that was bound to the double-stranded DNA on the electrode, and it can sense single base-pair mismatche within the sequence of the hybridized double-stranded DNA (dsDNA).

Info:

Periodical:

Advanced Materials Research (Volumes 301-303)

Edited by:

Riza Esa and Yanwen Wu

Pages:

213-218

DOI:

10.4028/www.scientific.net/AMR.301-303.213

Citation:

C. Zhou et al., "Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)32+", Advanced Materials Research, Vols. 301-303, pp. 213-218, 2011

Online since:

July 2011

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Price:

$35.00

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