Elimination of Vibrio parahaemolyticus Contamination in Shucked Oysters (Crassostrea plicatula) to with Natural Antimicrobial Agents TeaPolyphenols

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In this study, the survival of Vibrio parahaemolyticus in suspension in the presence of tea-polyphenols for 6h was first examined. The shucked oysters containing V. parahaemolyticus then were exposed to 2048μg/mL , 1024 μg/mL and 512 μg/mL tea-polyphenols for 4h and the population of V. parahaemolyticus in shucked oysters was determined every 1h. In addition, shucked oysters containing V. parahaemolyticus exposed into the dose of 1024 μg/mL tea-polyphenols were held at various temperature (0, 5, and 20°C) to examine survival of V. parahaemolyticus every 6h till 24h. Populations of V. parahaemolyticus in oyster were determined using 3-tube most probable number (MPN) method. The study found that the MIC of tea-polyphenols to Vibrio parahaemolyticus suspension is 1024 μg/mL; when exposed to 2048 μg/mL, 1024 μg/mL and 512 μg/mL tea-polyphenols for 4h, the population of V. parahaemolyticus in shucked oysters decreased by 3.29,2.43 and 1.84 Log10MPN/g respectively; 0, 5 and 20°C, 1024 μg/mL tea-polyphenols can decrease the population of V. parahaemolyticus in shucked by 4.10, 3.32 and 3.00Log MPN/g at 24th h. Sensory analysis showed that treatments of tea-polyphenols at concentrations 1024μg/mL did not cause negative effects on taste of oyster meat. These results indicated that tea-polyphenols have bactericidal effects against V. parahaemolyticus and can be applied to shucked oysters to reduce contamination of V. parahaemolyticus.

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Periodical:

Edited by:

Jun Hu and Qi Luo

Pages:

427-433

DOI:

10.4028/www.scientific.net/AMR.320.427

Citation:

X. S. Shen et al., "Elimination of Vibrio parahaemolyticus Contamination in Shucked Oysters (Crassostrea plicatula) to with Natural Antimicrobial Agents TeaPolyphenols", Advanced Materials Research, Vol. 320, pp. 427-433, 2011

Online since:

August 2011

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$35.00

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