Study on the Novel Label Free Impedimetric Immunosensor for Aflatoxin B1 Based on Poly(o-phenylenediamine) Film Modified Gold Electrode

Ning Ning Wu; Li Xin Cao; Pei Sheng Yan; Ming Hao Wang

doi:10.4028/www.scientific.net/AMR.322.385

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 322Study on the Novel Label Free Impedimetric...

Study on the Novel Label Free Impedimetric Immunosensor for Aflatoxin B1 Based on Poly(o-phenylenediamine) Film Modified Gold Electrode

Abstract:

A label free impedimetric immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing anti-AFB1 onto Poly o-phenylenediamine (PoPD) electropolymerized film modified gold electrode by glutaraldehyde (GA) cross-linking. An electrochemical interfacial modeling of biomolecular recognition was recommended and reasonably interpreted. EIS technology was employed for the quantitative determination. The linear detection concentration ranges of AFB1 were 0.03~0.1 μg/mL and 0.1~0.7 μg/mL. The detection limit was 2.8×10^-8μg/mL. The immunosensor could be reused more than 10 times when renewed by HCl-Glycine buffer solution (pH 2.6).

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Periodical:

Advanced Materials Research (Volume 322)

Pages:

385-389

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.322.385

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Online since:

August 2011

Authors:

Ning Ning Wu, Li Xin Cao, Pei Sheng Yan, Ming Hao Wang

Keywords:

Aflatoxin B1, Electrochemical Impedance Spectroscopy (EIS), Immunosensor, Poly-o-phenylenediamine

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