An increasing number of manufacturing enterprises commence to adopt flexible assembly line to meet the demands of the rapidly changing market. RFID technology is employed to collect the real-time production information in workshop and overcome difficulty to identify key production objects. In this paper, based on the RFID tag data model of smart objects, an RFID application solution on flexible assembly line is proposed, and an RFID-enabled manufacturing executing system is designed, which is integrated with the RFID-enabled real-time data capture system for flexible assembly line. In addition, the framework and integrated operation mode of corresponding RFID-enabled MES are established. The results of application in a motorcycle assembly line show obvious improvement in both production efficiency and quality.