Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector

Abstract:

Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider flagelliform silk expression cassette. Via antibiotic selection, most cells showed stable DsRed-expression. Immuno blot analysis showed that flagelliform silk protein of spider was expressed stably in BmN4 cells. Circular dichroism spectra indicated the existence of β-turn structure in recombinant spider flagelliform protein. The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.

• Periodical: Advanced Materials Research (Volumes 332-334)
• Pages: 779-782
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.332-334.779
• Online since: September 2011
• Authors: Yuan Song Zhang, Tian Fu Zhao, Ai Chun Zhao, Masao Nakagaki
• Keywords: Bombyx mori, Piggybac, Spider Flagelliform Silk, Transgenic

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