Mutagenesis, Screening and Industrialization of High Avermectin B1a Producing Strains from Streptomyces Avermitilis Irradiated by 12C 6+ Ion Beam

Abstract:

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Heavy ion irradiation induced mutation is a unique method of physical mutation, with various parameters, high LET and RBE characteristic. The spore suspension of streptomyces avermitilis were irradiated by carbon ions with doses of 0、30、40、50、60 and 70 Gy. The high avermectin producing strain was successfully obtained after the mutagenesis processing by heavy ion beam and the screening of orthomutation strains. Two strains(No.203 and No.148) which potency was 5200μg/ml was sieved and its potency increased 38.64% than the original strain. The obtained strains were used for 1 ton fermentation tank and the pilot 5-ton fermentation tank amplification tests.

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Periodical:

Edited by:

Zhijiu Ai, Xiaodong Zhang, Yun-Hae Kim and Prasad Yarlagadda

Pages:

652-655

DOI:

10.4028/www.scientific.net/AMR.339.652

Citation:

S. Y. Wang et al., "Mutagenesis, Screening and Industrialization of High Avermectin B1a Producing Strains from Streptomyces Avermitilis Irradiated by 12C 6+ Ion Beam", Advanced Materials Research, Vol. 339, pp. 652-655, 2011

Online since:

September 2011

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$35.00

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