Mutagenesis, Screening and Industrialization of High Avermectin B1a Producing Strains from Streptomyces Avermitilis Irradiated by ¹²C ⁶⁺ Ion Beam

Abstract:

Heavy ion irradiation induced mutation is a unique method of physical mutation, with various parameters, high LET and RBE characteristic. The spore suspension of streptomyces avermitilis were irradiated by carbon ions with doses of 0、30、40、50、60 and 70 Gy. The high avermectin producing strain was successfully obtained after the mutagenesis processing by heavy ion beam and the screening of orthomutation strains. Two strains(No.203 and No.148) which potency was 5200μg/ml was sieved and its potency increased 38.64% than the original strain. The obtained strains were used for 1 ton fermentation tank and the pilot 5-ton fermentation tank amplification tests.

• Periodical: Advanced Materials Research (Volume 339)
• Pages: 652-655
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.339.652
• Online since: September 2011
• Authors: Shu Yang Wang, Ji Hong Chen, Wen Jiang Li, Jian Ping Liang, Yong Heng Bo, Xiao Qi Ma, Jing Liu
• Keywords: Industrialization, Ion Beam of ¹²C⁶⁺, Irradiation, Screening, Streptomyces Avermitilis

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