Study of Non-Viral Gene Transfer of NB4 Cells

Abstract:

Post-Transcriptional Gene Silencing and RNA interference (RNAi) are terms describing the specific suppression of genes by complementary dsRNA. High-efficiency transfection is an essential first step for achieving effective gene knockdown. Non-viral transfection include chemical-transfection and electrotransfection. For a particular cell line, select the appropriate transfection method is very important. In this study, we compared a variety of commercially available reagents and electrotransfection, approaches to define methods for efficient delivery of siRNA to NB4 cells. Fluorescently-labelled siRNA were tested. We report that different methods have different effects. The results provide guidelines for optimal siRNA-mediated knockdown in the NB4 cells.

• Periodical: Advanced Materials Research (Volumes 341-342)
• Pages: 333-337
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.341-342.333
• Online since: September 2011
• Authors: Ying Chen, Huai Yu Wang, Mei Zhang
• Keywords: NB4 Cell, RNAi, Transfection

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