Screening of Lipid High Producing Mutant from Rhodotorula Glutinis by Low Ion Implantation and Study on Lipid Extration Technique

Abstract:

The paper studied on parameters of ion implantation into lipid producing strain Rhodotorula glutinis and lipid extration technology. It was found that the strain had a higher positive mutation rate when the output power was 10keV and the dose of N+ implantation was 80×2.6×1013 ions/cm2. Then a high-yield mutant strain D30 was obtained and it’s lipid yield which was 3.10 g/L increased by 33.05% than that of the original. Additionally, statistically-based experimental designs were applied for the optimization of lipid extraction by acid-heating coupling ultrasonic technique. By a Plackett-Burman design, it was found that three factors, treatment time of HCl (p=0.036) , ultrasonic time (p=0.0105) and rate of extracting solution (VCHCl3:VCH3OH) (p=0.0105), had significant effect on lipid extraction. Subsequently, these significant factors were optimized using response surface methodology (RSM), and the optimized parameters of lipid extraction were as follows: 34 min for treatment time of HCl, 7.5 min for ultrasonic treating time, 1.9:1 for rate of extracting solution (VCHCl3:VCH3OH). Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 d, it’s lipid yield increased to 7.81 g/L

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 1172-1181
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.1172
• Online since: September 2011
• Authors: Shi Chang Li, Hong Xia Liu, Shao Bin Gu, Min Wang, Zhao Yang Zhu, Dong Dong Wang
• Keywords: Ion Implantation, Lipid Extraction, Plackett-Burmen Design, Response Surface Method (RSM), Rhodotorula Glutinis

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