In this study, the microbial degradation characteristics of butyl benzyl phthalate (BBP) were studied by using Phanerochaete chrysosporium. Results showed Phanerochaete chrysosporium can effectively degrade BBP and the degradation efficiency can reach 90% in 2 days. This strain can use BBP as sole carbon source. The biodegradation of BBP by Phanerochaete chrysosporium fits for first-order kinetics (lnc=-Kbt+a), which are lnc = -0.0445x + 4.4235、lnc = -0.0404x + 5.1221、lnc =-0.0389x + 5.6363, respectively, for the concentration of 100mg/L, 300mg/L, 500mg/L. The biodegradation rate constants are 0.0445 d-1, 0.0404 d-1, 0.0389 d-1, respectively. The biodegradation rate constant reduces with the increase of the concentration of BBP, indicating BBP has inhibitory effect on Phanerochaete chrysosporium, especially under high concentration. The high performance liquid chromatography (HPLC) profile of the metabolites indicated the accumulation of two major products except for phthalic acid (PA). These two products were characterized by high performance liquid chromatography–mass spectrometry (HPLC/MS) as mono-n-butyl phthalate (MBuP) and monobenzyl phthalate (MBzP).