Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants

Shao Jiong Zhong; Wei Qi; Hai Kuan Wang

doi:10.4028/www.scientific.net/AMR.343-344.551

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 343-344Strain Improvement of Acinetobacter Calcoaceticus...

Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants

Abstract:

A strain improvement program was developed to increase lipase production by Acinetobacter calcoaceticus D29, in which mutants were generated by Ultraviolet (UV) radiation and selected by resistance to 1.5% (w/v) sodium citrate. One of the mutant strains UN9 exhibited 48.69% more lipase activity than the original strain did. Although the UV random mutagenesis alone is an effective method for general strain improvement, our results showed that UV mutagenesis combined with selection for sodium citrate resistance is a more efficient strategy for screening mutant strains with enhanced lipase production.

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Advanced Materials Research (Volumes 343-344)

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551-555

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https://doi.org/10.4028/www.scientific.net/AMR.343-344.551

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September 2011

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Shao Jiong Zhong, Wei Qi, Hai Kuan Wang

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Acinetobacter Calcoaceticus, Lipase, Resistant Mutagenesis, Sodium Citrate, UV

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