Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants

Abstract:

Article Preview

A strain improvement program was developed to increase lipase production by Acinetobacter calcoaceticus D29, in which mutants were generated by Ultraviolet (UV) radiation and selected by resistance to 1.5% (w/v) sodium citrate. One of the mutant strains UN9 exhibited 48.69% more lipase activity than the original strain did. Although the UV random mutagenesis alone is an effective method for general strain improvement, our results showed that UV mutagenesis combined with selection for sodium citrate resistance is a more efficient strategy for screening mutant strains with enhanced lipase production.

Info:

Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

551-555

DOI:

10.4028/www.scientific.net/AMR.343-344.551

Citation:

S. J. Zhong et al., "Strain Improvement of Acinetobacter Calcoaceticus D29 for Enhanced Lipase Production by Screening Sodium Citrate Resistant Mutants", Advanced Materials Research, Vols. 343-344, pp. 551-555, 2012

Online since:

September 2011

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.