Cloning and Characterization of Chicken CRBP2 Gene and Comparison with other Vertebrates

Abstract:

Cellular retinol-binding protein 2 (CRBP2), a vitamin A binding protein expressed specifically in small intestinal villus absorptive cells, plays a pivotal role in the intestinal vitamin A absorption, transport, and metabolism pathways. In this study, we cloned the entire coding region of chicken CRBP2 gene. The amplified fragment contains entire coding region sequence with 408 nucleotides, which putatively codes 135 AA. By comparing nine vertebrates, the homology of nucleotide sequences is from 52.3% to 99.8%, while the similarity of AA sequence ranged from 72.4% to 99.3%. Results showed that the CRBP2 gene was conservative among different animal species. This work constructed the basis for further research on the molecular nature and genetic markers of CRBP2 for improving egg production traits in chicken.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 678-682
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.678
• Online since: September 2011
• Authors: Li Hua Xiao, Fan Li Kong, Hua Dong Yin, Xiao Ling Zhao, Qing Zhu
• Keywords: Cloning, CRBP2, Sequence Analysis

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