Optimization of the Sporogenous Medium of Irpex Lacteus Using Response Surface Methodology

Abstract:

Response surface methodology (RSM) was used to optimize sporulation medium components for improving spores concentration in I. lacteus fermentation broth. Five factors of sporulation medium components, glucose (X₁), peptone (X₂), yeast extract (X₃) MgSO₄·7H₂O (X₄) and VB₁ (X₅), were chosen to optimize through a five-level, five-factor central composite design (CCD) base on single factor experiments. The concentration of medium components which influenced the sporulation of I. lacteus was further optimized using RSM. The results show that The medium that consists of 4.15 g/L peptone, 2.04 g/L yeast extract, 5.10 g/L glucose, 0.17 g/L MgSO₄·7H₂O and 0.03 g/L VB₁ enhance spores concentration significantly . The final spores concentration (2.19×10⁵ /mL) by using the optimized medium was about five times higher than normal medium.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 832-837
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.832
• Online since: September 2011
• Authors: Na Zhang, Qing Yan Chen, Ruo Chuan Pan, Xue Feng Yao, Yan Feng Wang, Jia Hui Lu, Li Rong Teng
• Keywords: Fermentation, L. lacteus, Medium Optimization, Response Suface Methodology (RSM), Sporulation

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