For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Study on Rational Deployment of Dewatering Depressurization Project of the Cambrian Limestone Geothermal Water
p.1510
The Crisis in Urban Water Environment and Land Use
p.1518
The Experimental Research Based on SHPB Test of the Rocks Plastic Damage Cumulative Effect
p.1523
The Numerical Investigation on Optical Efficiency and Heat Flux Distribution of Conical Cavity Receiver
p.1530
Development of an Analytical Protocol for the Determination of Polybrominated Biphenyls in Water by Enzyme-Linked Immunosorbent Assay
p.1537
Study on Solar Desalination with Enhanced Falling Film Evaporation
p.1542
The Method of Material Flow Analysis about Circular Economy in Coal Mining Area
p.1549
FeedWater System Transient Analysis of Nuclear Power Station
p.1557
Key Technologies of Rebuilding Underground Natural Gas Storages from Carbonate Buried Hill Gas Reservoirs
p.1561

Development of an Analytical Protocol for the Determination of Polybrominated Biphenyls in Water by Enzyme-Linked Immunosorbent Assay

Article Preview

Abstract:

A novel immunoassay has been developed for the quantitative determination of polybrominated biphenyls (PBBs) using indirect competitive format. A new method was developed to synthesize PBBs congener (PCB15) hapten and its artificial immunogen, then the polyclonal antibodies. The assay was optimized concerning the coating conjugate and antibody concentration, incubation time and temperature, the tolerance to organic solvents and so on. Under optimized conditions, PBB15 can be determined in the concentration range of 0.01-100 μg L-1 with a detection limit of 0.02 μg L-1. The cross-reactivities of the assays were below 8%. While water samples could be analyzed directly.

You might also be interested in these eBooks
Renewable and Sustainable Energy View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 347-353)

Pages:

1537-1541

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.347-353.1537

Citation:

Cite this paper

Online since:

October 2011

Authors:

Han Yu Chen, Hui Sheng Zhuang

Keywords:

Antigen, Immunoassays, Polybrominated Biphenyls, Polyclonal Antibody

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2012 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] M.F. Fernandez, P.Araque, H.Kiviranta, J.M. Molina-Molina :Chemosphere Vol.66(2007), p.377.

Google Scholar

[2] Y.H. Yang, Y.S. Chang, B.H Kim: Chemosphere Vol.47(2002), p.1087.

Google Scholar

[3] J.Gieron´, A. Grochowalski, R. Chrzaszcz: Chemosphere Vol.78 (2010) , p.1272.

Google Scholar

[4] T.Hyoëtylaëinen, K. Hartonen: J. Trends.Anal.Chem Vol. 21 ( 2002), p.13.

Google Scholar

[5] V.W. Burse, L.L. Needham: J. Anal. Toxicol Vol.4(1980), p.22.

Google Scholar

[6] B.G´omara, G.R. Carmen, , J.Mar´ıa, L. M. J.Mar´ıa: Anal. Chim. Acta Vol. 565(2006), p.208.

Google Scholar

[7] P.Koryt´ar, P.E.G. Leonards, J.Boer de, U.A.Th.Brinkman:J. Chromatography A Vol. 1086(2005), p.29.

Google Scholar

[8] M. Fránek, A.P. Deng, V. Koláˇr:Anal. Chim. Acta Vol. 444(2001), p.131.

Google Scholar

[9] C.J. Chuang, L.S. Miller, D.B. Davis: J. Anal. Chim. Acta Vol.376(1998), p.67.

Google Scholar

[10] T. Tomoaki, A. Yoshiaki, A.Okuyama: Chemosphere Vol.65(2006), p.467.

Google Scholar

[11] P.N. Nuria, M. Sergi, M. Angel, P. Rosa: Anal. Chim.Acta Vol.594 (2007) 211.

Google Scholar

[12] Y.Y Yu, Q.E. Wang, H.S. Zhuang: J. Anal. Lett. Vol.39(2006), p.937.

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.