Determination of Doxycycline Residues in Milks and Content in Serum, Tablet and Injection Samples with Self-Ordered Ring Fluorescence Microscopic Imaging Technique

Abstract:

A new method of determinating doxycycline residues in milks and content in serum,table and injection samples was established with Self-ordered ring fluorescence microscopic imaging technique. In the presence of hexahydropyridine and poly (vinyl alcohol)-124, Zn²⁺-doxycycline (DC) system can form a SOR on the hydrophobic glass slides surfaces based on the capillary effect. The maximum fluorescent intensity (I_max) at central ring belt was found to be proportional to DC content. when the droplet volume is 0.5μL, the present SOR method can be used to determine DC in a range of 4.54×10^-15–6.81×10^-13 mol•ring^-1, and the limit of detection (LOD) with a threefold signal to noise ratio (S/N = 3) was 4.54×10^-15 mol•ring^-1 (2.17×10^-8 mol•L^-1). With the present method, the residues in milks and content in serum, tablet and injection samples were satisfactorily detected with recoveries of 96.3-102.0% and RSD of 1.4-2.2%, respectively, indicating that the method is reliable and practical.

• Periodical: Advanced Materials Research (Volumes 347-353)
• Pages: 2356-2359
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.347-353.2356
• Online since: October 2011
• Authors: Ying Liu, Yan Min Yu, Yan Wang
• Keywords: Doxycycline, Residue, Self-Ordered Ring Fluorescence Microscopic Imaging Technique

References

[1] Y.L. Zhang, S.X. Lu, L. Wei. J. Agric. Food Chem. 55 (2007) 211-218.

[2] G. P. Allena, L. M. Deshpande. Int J Antimicrob Ag. 35 (2010) 45-49.

[3] A. L. Saber, A. S. Amin. J. Anal. Bioanal.Techniques. 1 (2011) 113.

[4] D. Farin, G. Piva, I. Gozlan, R. Kitzes. Chromatographia. 47 (1998) 547-549.

[5] T. Le, H. Yu, Y. C Guo, B. Ngom, Y. A. Shen, D. R. Bi. Food Agr. Immunol. 20 (2009) 111-124.

[6] P. J. Ramesh, K. Basavaiah, M. R. Divya. J.Anal.Chem. 66 (2011) 482-489.

[7] W. H.Liu, Y. Wang, J. H. Tang, G. L. Shen, R. Q. Yu. Analyst, 123 (1998), 365-369.

[8] Y. Liu, C. Z. Hang, Y. F. Li. Anal. Chem. 74(2002) 5564-5568.

[9] C. Z. Hang, Y. Liu. Y. F. Li. J. Pharm. Biomed. Anal. 34(2004) 103-114.

[10] Y. Liu, Y. F. Li. C. Z. Hang. J. Anal. Chem. 61(2006) 647-653.

[11] S. M. Sunaric, S. S. Mitic, G. Z. Miletic, A. N. Pavlovic, D. Naskovic-Djokic. J.Anal.Chem. 64 (2009) 231–237.

DOI: 10.1134/s1061934809030046

[12] D. Farin, G. Piva, I. Gozlan, R. Kitzes. Chromatographia. 47(1998) 547-549.

[13] Y. B. Zhao, D. Y. Wang, J. G. Xu. J. Instrum. Anal (In Chinese). 15 (1996) 77.