Molecular Cloning and Characterization Analysis of 3,8-Divinyl Protochlorophyllide a 8-Vinyl Reductase Gene from Dunaliella parva
The vast majority of photosynthetic organisms utilize monovinyl chlorophyll for their photosynthetic reactions. For the biosynthesis of monovinyl chlorophyll, the reduction of the 8-vinyl group which is located on the B-ring of the macrocycle is essential. 3,8-Divinyl protochlorophyllide a 8-vinyl reductase (DVR) catalyzes the reduction of 8-vinyl group on the tetrapyrrole to an ethyl group, which is necessary for monovinyl chlorophyll (Chl) synthesis. The former studies indicated the DVR could enhance photosynthesis. The full-length cDNA encoding DVR was obtained from oleaginous microalgae Dunaliella parva, which include 1326 bp open reading frame (ORF), 22 bp 5′-untranslated sequence and 383 bp 3′-untranslated sequence. Dunaliella parva DVR showed the highest sequence similarity with the DVR from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva DVR also showed wide similarity with other species.
Weiguo Pan, Jianxing Ren and Yongguang Li
C. H. Shang et al., "Molecular Cloning and Characterization Analysis of 3,8-Divinyl Protochlorophyllide a 8-Vinyl Reductase Gene from Dunaliella parva", Advanced Materials Research, Vols. 347-353, pp. 3203-3206, 2012