This document discussed a biofilter packing with complex filler, which was inoculated by a kind of toluene-degrading microorganism. The toluene-degrading microorganism(JBJ-3), which was isolated from activated sludge from a wastewater treatment plant of refinery, was classiﬁed as Pseudomonas sp. initially by physiological and biochemical tests. The removal efficiency of JBJ-3 reached to 93.73% for 48h. Complex filler consisted of wood chip, peat and compost etc. It has high amounts of organic matter, high speciﬁc surface area, good water holding capacity, good permeability, varied microbial system, and contains intrinsic nutrients. The complex filler could inoculate Pseudomonas sp. JBJ-3 well. And under the different inlet concentrations of toluene, the overall removal efficiency had reached above 92%. And the bioﬁlter packing complex filler showed a good resistance to shock load.