Isophthalonitrile(IPN) used in this study contains two cyano groups(-CN) attached to the m-benzene ring. The IPN wastewater is degraded by biological method of high efficiently coupling microorganism. The choice of carrier is the key factor of immobilized biological technology. Two immobilization methods, adsorption with ceramic as the carrier，embedding with Sodium Alginate(SA) and Polyvinyl Alcohol –Sodium Alginate(PVA-SA) as the carrier, the research is conducted into the degradation effects. Results showed that IPN biodegradation by adsorption method had the best effect degrading. The IPN degradation rate kept stable and was over 96%. Additionally, in order to demonstrate purification of the IPN wastewater. The laboratory-scale experiment was carried out in a biological aerated filter (BAF) that was filled with ceramic filler. Results also showed that the BAF reactor removed the IPN and the rate was over 95%.