Rapid Determination of 5-Hydroxymethylfurfural by Ultraviolet Spectrophotometry in Glucose Diphasic Hydrolysate

Liu Yang; Yu Huan Liu; Rong Sheng Ruan

doi:10.4028/www.scientific.net/AMR.361-363.1713

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 361-363Rapid Determination of 5-Hydroxymethylfurfural by...

Rapid Determination of 5-Hydroxymethylfurfural by Ultraviolet Spectrophotometry in Glucose Diphasic Hydrolysate

Abstract:

The content of 5-hydroxymethlfurfural (5-HMF) in glucose diphasic hydrolysate was measured by ultraviolet spectrophotometry (UV), while 5-HMF solution and distilled water was used as the standard sample and controlling blank instead of water-dimethylsulfoxide (DMSO) diphasic solution respectively, analyzing the content of 5-HMF at wavelength 284 nm. The determination results showed us a favorable linear relationship between the absorbance and the concentration of 5-HMF, when it is fall in between 0-0.10 mmol/L. This method is suitable for rapid and accurate determine 5-HMF.

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Periodical:

Advanced Materials Research (Volumes 361-363)

Pages:

1713-1717

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.361-363.1713

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Online since:

October 2011

Authors:

Liu Yang, Yu Huan Liu, Rong Sheng Ruan

Keywords:

5-Hydroxymethlfurfural(5-HMF), Glucose Diphasic Hydrolysate, Ultraviolet Spectrophotometry (UV)

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