Rapid Determination of 5-Hydroxymethylfurfural by Ultraviolet Spectrophotometry in Glucose Diphasic Hydrolysate
The content of 5-hydroxymethlfurfural (5-HMF) in glucose diphasic hydrolysate was measured by ultraviolet spectrophotometry (UV), while 5-HMF solution and distilled water was used as the standard sample and controlling blank instead of water-dimethylsulfoxide (DMSO) diphasic solution respectively, analyzing the content of 5-HMF at wavelength 284 nm. The determination results showed us a favorable linear relationship between the absorbance and the concentration of 5-HMF, when it is fall in between 0-0.10 mmol/L. This method is suitable for rapid and accurate determine 5-HMF.
Qunjie Xu, Honghua Ge and Junxi Zhang
L. Yang et al., "Rapid Determination of 5-Hydroxymethylfurfural by Ultraviolet Spectrophotometry in Glucose Diphasic Hydrolysate", Advanced Materials Research, Vols. 361-363, pp. 1713-1717, 2012