Extraction of amygdalin by ethanol refluxing from waste loquat kernel was performed successfully. Amygdalin concentrations were determined by using ultraviolet spectrophotometry with ethanol as solvent at 210 nm. And the influences of various factors on extraction process were discussed. The result shows that the concentration of ethanol is the most influencing factor in this process, and then the solid-liquid volume ratio, the extraction temperature and the extraction time. Optimum conditions for this extraction process are: the solid-liquid volume ratio is 1/10, the concentration of ethanol is 65%, the extraction temperature is 60°C and the extraction time is 45min.