Preliminary Study on Inducing Incompact Callus of Polygonum cuspidatum Using the Medium with Perlite

Abstract:

.Preparation of the suspension cells of Polygonum cuspidatum needed the incompact calli by inducing the explants, but the calli from Polygonum cuspidatum reported, always were too tight to fit for obtaining a lot of the single cells or small cell aggregations. In this paper, we used its stem apex as the explant to inducing the calli in the medium containing agar or perlite, respectively, and analyzed the calli development ratio, the calli texture property and the calli differentiation. The results indicated that, compared with the agar, the calli induced developed more rapidly in the medium using the perlite; the suspension cells were more easily gotten from the calli, and their cell aggregations were well dispersed and little grown into the differentiated tissues. As a consequence, the perlite, instead of the agar, may be used as a solid component of the medium to induce incompact callus of Polygonum cuspidatum.

• Periodical: Advanced Materials Research (Volume 365)
• Pages: 140-144
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.365.140
• Online since: October 2011
• Authors: Xing Lin Li, Dan Dan Wang, Jie Gao, Xing Hua Cui, Jun Zhao, Li Ming Zhang, Wen Yuan Gao, Lan Xiao
• Keywords: Cell Aggregation, Cell Suspension Culture, Incompact Callus, Perlite, Plant Hormone Combination, Polygonum cuspidatum

References

[1] Liu S, Cheng L, Exploitation actuality and expectation of polygonum cuspidatum's effective ingredients, Food Science and Technology, vol. 2(2), p.96－98, (2005).

[2] Meishiang J, Lining C, Udeani G, Cancer chem opreventive activity of resveratrol, a natural product derive from gapes, Science，vol. 275(1), p.218－220, (1997).

[3] Hisashi M, Hiroshi S, Toshio M, Phytoestrogens from the roots of Polygonum cuspidatum (polygonaceae)：structure–requirement of hydroxyanthraquinones for estrogenic activity, Bioorganic and Medicinal Chemistry Letters, vol. 11(4), p.1839－1842, (2001).

DOI: 10.1016/s0960-894x(01)00318-3

[4] Cheol S P, Yonng C L, Jong D K, et al, Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component resveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells, Vascular Pharmacology, vol. 40(6), p.279－284, (2004).

DOI: 10.1016/j.vph.2004.01.003

[5] Wang S, Zheng Z, Weng Y, Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts, Life Sciences, vol. 74(20), p.2467－2478, (2004).

DOI: 10.1016/j.lfs.2003.03.005

[6] Zhou J, Zhang H, Yang P, Li H, Determination of resveratrol glucoside and resveratrol in radix and rhizome of Polygonum cuspidatum yielded in Hanzhong Region, Chinese Traditional and Herbal Drugs, vol. 33(5), p.414－416, (2002).

[7] Wang Z, Luo Z M, Deng L W, The Pharmacological Mechanism and Synthesis of Resveratrol, Natural Product Research and Development，vol. 15(2), p.178－181, (2003).

[8] Wang T M, Wang X M, Recent Progress of Pharmacological Functions of Polydatin, Journal of Suzhou College，vol. 22(2), pp.114-116, (2007).

[9] Cao Y, Chen X X, Tang Y H, Xiang H, et al, Study on High Produce Resveratrol Material Filtration and Callus Abduction of Polygonum cuspidatum, Life Science Research, vol. 10(3), p.271－275, (2006).

[10] Yang P J, Li H N, Zhao H, Tissue culture and rapid propagation of Reynoutria japonica, Acta Botanica Boreali-occidentalia Sinica, vol. 23(12), p.2192－2195, (2003).

[11] Wen T, Liang L, Zeng Y, Yu X, Effect of different light intensity on Polygonum cuspidatum callus, China Journal of Chinese Materia Medica, vol. 32(13), p.1277－1280, (2007).

[12] Ou J Q, Chen X X, Ding L H, Cao Y, Callus Inducement and Resveratrol Formation of Polygonum cuspidatum, Journal of Central South Forestry University，vol. 26(3), p.24－27, (2006).

[13] Yi A Q, Tong F P, Song Q, et al, The tissue culture technology of Polygonum cuspidatum sieb et zucc, Hunan Forestry Science & Technology，vol. 34（1）, p.10－12, (2007).

[14] Jiang S, Zhu R R, Jing S L, et al, Optimization of extraction of polydatin from Polygonum cuspidatum, Chinese Journal of New Drugs, vol. 17(22), p.1953－1956, (2008).

[15] Xu H L, Fu Y H, Gao P, Xu C J, Study on Extraction Process of Resveratrol in Polygonum cuspidatum, Lishizhen Medicine and Materia Medica Research，vol. 17(8), p.1424－1425, (2006).

[16] Xiang H H, Dai K J, Luo Q Z, Optimization of extraction process for resveratrol from Polygonum cuspidatum Sieb et Zucc after enyzmatic treatment, Journal of Central South University (Science and Technology) , vol. 39(4), p.700－704, (2008).

[17] Jun I, Suzuki, Growth dynamics of shoot height and foliage structure of a rhizomatous perennial herb，Plygonum cuspidatum, Annales Botany，vol. 73(6), p.629－638, (1994).

DOI: 10.1006/anbo.1994.1079