Preliminary Study on Inducing Incompact Callus of Polygonum cuspidatum Using the Medium with Perlite
.Preparation of the suspension cells of Polygonum cuspidatum needed the incompact calli by inducing the explants, but the calli from Polygonum cuspidatum reported, always were too tight to fit for obtaining a lot of the single cells or small cell aggregations. In this paper, we used its stem apex as the explant to inducing the calli in the medium containing agar or perlite, respectively, and analyzed the calli development ratio, the calli texture property and the calli differentiation. The results indicated that, compared with the agar, the calli induced developed more rapidly in the medium using the perlite; the suspension cells were more easily gotten from the calli, and their cell aggregations were well dispersed and little grown into the differentiated tissues. As a consequence, the perlite, instead of the agar, may be used as a solid component of the medium to induce incompact callus of Polygonum cuspidatum.
X. L. Li et al., "Preliminary Study on Inducing Incompact Callus of Polygonum cuspidatum Using the Medium with Perlite", Advanced Materials Research, Vol. 365, pp. 140-144, 2012