In recent years, variety of environmentally friendly approaches has been studied for effectively controlling biofouling on marine structures. Research on shells surfaces that inhibit marine biofouling has attracted the attention of people. In this study, we take Dosinia japonica as biomimetic object, PDMS as negative material and E44 epoxy resin as positive material. The method of vacuum mixing and casting in zip lock bag was used to obtained biomimetic surface with the microstructure of shell surface. The microstructure of corresponding position was compared by using high resolution digital camera. The LI-3 contact surface profilometer was used to measure its surface morphology and then the data was processed through MATLAB wavelet analysis. Furthermore, the automatic contact angle measuring instrument was adopted to measure the contact angle for comparison. The results show that there are high similarities in multi-scale between resin biomimetic surface and Dosinia japonica surface. We accurately copied the microscopic structure of the shell surface whose hydrophobic performance was slightly better than the shells.