Improved Enzymatic Depolymerization of Chitosan by Ionic Liquid Pretreatment and the Effect of Oligo-Chitosan on Intestinal Flora In Vitro

Abstract:

It still remains challenging for effective hydrolysis of chitosan into chitosan oligomers. In this work, a pretreatment was conducted on chitosan by an ionic liquid 1-butyl-3-methylimidazolium chloride ([C4mim]Cl), aiming at improving enzymatic depolymerization of chitosan. X-ray diffraction analysis indicated that the inter- and intra-molecular hydrogen bonds within chitosan molecules were broken by [C4mim]Cl and the crystalline was destroyed. The oligo-chitosan hydrolyzed from IL-pretreated chitosan, coded as COS-IL, showed a DP of 3~5, in contrast to DP 5~8 with oligo-chitosan obtained from untreated chitosan(coded as COS-UN). COS-IL was more effective than COS-UN in inhibiting intestinal spoilage bacterials growth and it has positive effect on the growth of intestinal probiotic bacterials.

• Periodical: Advanced Materials Research (Volumes 391-392)
• Pages: 1319-1323
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.391-392.1319
• Online since: December 2011
• Authors: Cui Zheng, Lin Li, Hao Pang, Zhao Mei Wang, Na Li
• Keywords: Enzymatic Depolymerization, Intestinal Flora, Ionic Liquid (IL), Oligo-Chitosan

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