Color Development Reaction Research of Melamine in Milk

Abstract:

According to the color development reaction between starch/iodine reagent and products which obtained by fumigating melamine with chlorine, a method for determination of melamine in milk was put forward. The conditions of method were researched. The best conditions are as follows. Volume ratio of milk and extraction agent is 1:1. 5, dissolved with ultrasonic washer for 5 minutes, centrifugal separation 4000r/min for 5 minutes, extracted by C008 cation exchange resin column. The results are as follows: detection limit is 10mg/L, the linear range is 25-250 mg/L, related coefficient r is 0.994, coefficient of recovery is 94%-110%, and relatively standard deviation is 0.9%. This method is easy handling, cheap equipment, good practicability and recovery, can be used as low cost detection method.

• Periodical: Advanced Materials Research (Volumes 393-395)
• Pages: 1133-1138
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.393-395.1133
• Online since: November 2011
• Authors: Xi Quan Sun, Sheng Lin Qiao, Zheng Xiao Liu, Ling Le Kong
• Keywords: Color Development Reaction, Melamine, Milk, Starch/Iodine Reagent

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