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Extraction and Purification of Astaxanthin from Shrimp Shell with Alkaline-Organic-Solvent Method
Abstract:
The Alkaline-organic-solvent Method was employed to extract astaxanthin from shrimp shell in this work. The results demonstrated that the optimum extraction temperature was 20°C, the optimum ratio of extraction agent to sample is 1:3. Centrifugal thin layer chromatography was used to purify the astaxanthin and the optimum developer was petroleum, acetone and methanol (14:5:1). The FT-IR spectra and the HPLC analysis both demonstrated the purified substance was identical with standard astaxanthin and the content of astaxanthin account for 55.4%.
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1231-1234
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November 2011
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© 2012 Trans Tech Publications Ltd. All Rights Reserved
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