Study on the Technology of Extraction Protein from Fish Swim Bladder with Ultrasonic Treatment

Abstract:

A fresh fish swim bladder was used to extract protein with ultrasonic treatment technology. The coomassie brilliant blue G-250 was used to determine the protein content. Effects of ultrasonic power, duty cycle and pH of extraction solution on the extraction rate of proteins were investigated in this paper. The conditions of extracting protein from swim bladder by ultrasonic treatment were optimized by orthogonal experiments. The results indicated that the optimized conditions were ultrasonic power 125W, duty cycle 70%, and the pH of solution 1.0 respectively. This provided a basis for the development of application of ultrasonic extraction technology in the processing of fish swim bladder.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 1413-1416
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.1413
• Online since: November 2011
• Authors: Zhen Hua Duan, Yan Lan Li, Ju Lan Wang
• Keywords: Extraction, Fish Swim Bladder, Protein, Ultrasonic Wave (UW)

References

[1] Z.H. Duan, A.Q. Yin and Q.L. Jia: Food Research and Development Vol. 27 (2006), p.136

[2] Z.H. Duan, J.L. Wang, A.Q. Yin:Food Research and Development Vol. 28 (2007), p.62

[3] P.Lea, V.H. Christoph:Analytical and Bioanalytical Chemistry Vol. 376 (2003), p.908

[4] K.J. Lee, K.H. Row: Korean Journal of Chemical Engineering Vol. 23 (2006), p.779

[5] S.H. Zhang: Food Analysis (China Light Industry Press, Beijing 2007).

[6] M.Bradford: Anal. Biochem Vol. 72 (1976), p.248

[7] L.Zeng, C.X. Lian and Z.N. Xia: Journal of Chongqing University Vol. 25 (2002), p.53