Function Analysis of Luciferase Reporter Plasmid with AQP1 Gene Promoter

Abstract:

The aquaporins (AQP) are a family of homologous water transporting proteins that are expressed in many epithelial, endothelial and other tissues. Myocardin is important for SMC differentiation, but its precise role in regulating the initiation of AQP1 transcription activity is less clear. Function analysis of AQP1 promoter luciferase reporter plasmid will provide the theory basis for researching the function of transcription activity. In this study, the rat and human myocardin promoter luciferase reporter construct were successfully constructed. Then to determine whether AQP1 transcription activity were regulated by myocardin, luciferase repoeter assays were performed in COS-7 cells. The results illustrated that myocardin significantly activated rat and human AQP1 promoter. AQP1 promoter transactivity was inhibited by △Q. The present study provided the first evidence that myocardin may have an influence on the expression of AQP1 and reveal a basis of the mechanism transcriptional regulation of AQP1.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 1486-1489
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.1486
• Online since: November 2011
• Authors: Yong Jiang, Zhe Sun, Tong Cun Zhang
• Keywords: AQP1, CArG Box, Luciferase Activity Assay, Promoter

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