Effect of MIG1 Gene Deletion on Glucose Repression in Baker’s Yeast

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Abstract:

Mig1p, a zinc finger class of DNA-binding protein, plays a critical role in glucose repression for maltose utilization in Baker’s yeast. Maltose is the hydrolyzate of starch, which is the main component of dough. In this study, the recombinant yeast strains with MIG1 gene deletion were constructed, and the maltose metabolism of the parental and mutant strains in batch cultivations were investigated. Our results show that the degree of glucose repression of mutants △MIG1α and △MIG1a are reduced by 49.88% and 41.59% respectively compared to their parental strains, suggesting that MIG1 deletion can partially relieve glucose repression of maltose metabolism.

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Periodical:

Advanced Materials Research (Volumes 396-398)

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1531-1535

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November 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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