Determination of Sugars in β-Amylase Hydrolysates by High Performance Liquid Chromatography

Abstract:

Abstract. The chromatographic conditions for the separation of glucose, maltose and maltotriose on a ZORBAX Eclipse XDB-C18 column (4 um, 4.6×250mm) by use of a 2414 differential refractometer detector were determined. The square of the correlation coefficient of glucose, sucrose, maltose and maltotriose was 0.9973, 0.9981, 0.9977 and 0.9987, respectively. When the ratio of signal and noise was 3, the detection limit of glucose, sucrose, maltose and maltotriose was 0.3, 0.3, 0.20, 0.20 mg/mL the β-amylase hydrolysates consisted mainly of maltose, and maltotriose next, glucose only in trace amount, and no sucrose. The RSD of glucose, maltose and maltotriose was 2.08%, 2.41% and 2.61%, respectively. And the recovery of glucose, maltose and maltotriose was 101.3%, 98.4% and 93.5%. It was credible to separate sugars from hydrolysates. The method is important for improving the inducible enzymes activity.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 1575-1578
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.1575
• Online since: November 2011
• Authors: Xin Hong Liang, Bin Li, Gang Li, Yu Tang, Zu Feng Guo
• Keywords: Glucose, High Performance Liquid Chromatography (HPLC), Maltodextrin, β-Amylase Hydrolysates

References

[1] M. R. Schmitt, L. Marinac. Journal of Cereal Science Vol. 47 (2008), p.480–488.

[2] H. A. Adeniran and S. H. Ogunsua. Food Bioprocess Technol Vol. 3 (2010), p.693–698.

[3] S. Schönert, S. Seitz and H. Krafft: J. Bact Vol. 188 (2006), p.3911–3922

[4] J. L. Sun, R. X. Zhao, X. H. Liang. Statistics Application in Management Science and Engineering, Vol. 1 (2010), pp.562-567.