Optimization of Fermentation Conditions for Synthesis Dextran by Biochemical Conversion

Abstract:

To improve the production of dextran, fermentation conditions for cultivation of an Leuconostoc mesenteroides CICC-21725 were investigated. The fermentation conditions were optimized preliminarily by using the method of univariate experiment in the shake-flask culture, and the yield rate of dextran was used as the investigation index. The result shows that when the fermentation temperature, initial pH value, rotation speed and inoculum concentration were 25 °C, 7.5 , 0 r/min and 10%, respectively, the yield rate of dextran was the highest. Under the optimal fermentation conditions, the yield rate is approximately 98%, which is about 12% higher than the unoptimized.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 1611-1614
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.1611
• Online since: November 2011
• Authors: Ming Zheng Liang, Mei Li, Li Hong Lan, Tao Xie, Lei Zhong, An Ping Liao
• Keywords: Biosynthesis, Fermentation Condition, Leuconostoc mesenteroides

References

[1] J.F. Robyt, B.K. Kimble and T.F. Walseth: Biochem.Biophys. Vol. 165 (1974), pp.634-640.

[2] A. Jeanes, W.C. Haynes, C.A. Wilham, J.C. Rankin, E.H. Melvin, M.J. Austin et al. J Am Chem Soc. Vol. 76 (1954), p.5041.

[3] N. Barbani, F. Bertoni, G. Ciardelli, et al.: European Polymer Journal, Vol. 41 (2005), p.3004.

[4] F. Lino, H.G. Maria, M.S. Antonio et al.: Biomaterials, Vol. 26 (2005), p.4707.

[5] M.H Tang, H.J. Dou and K. Sun: Polymer. Vol. 47 (2006), p.728.

[6] S.H. Nam, E.A. Ko, S.S. Jang, et al.: Biotechnol Lett, Vol. 30 (2008), p.135.