Quantification and Enrichment of Esculeoside a from Fresh Tomato Fruits

Abstract:

Esculeoside A, the tomato saponins from Lycopersicon esculentum MILL, may be metabolized into various steroidal hormones such as pregnane derivatives that are expected to exhibit various bioactivities in the body, such as anti-osteoporosis, antimenopausal disorder and anti-tumor actions. In order to enrich large amount of esculeoside A for further scientific research, we firstly developed a high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) method for its quantification, and used it to examined the content of esculeoside A in different cultivated varieties of tomato fruits and tomato extract. Statistical analysis of the obtained data demonstrated that our method was simple, and has achieved desired linearity, precision and accuracy. Then we have explored the feasibility of using pectinase as a means for assisting esculeoside A extraction from fresh tomato fruits, and several factors such as the enzyme concentration, pH value of enzyme solution, temperature, incubation time which influenced on extraction were investigated. Moreover, in our previous study, the adsorption characteristics on several types of macroporous resins have been compared. Among them, D-101 resin showed the best separation behaviors on esculeoside A. So in the present paper, in order to get the best parameters for D101 adsorption, the adsorption kinetic curve, the appropriate ethanol concentration for elution and breakthrough volume were analyzed．

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 556-564
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.556
• Online since: November 2011
• Authors: Feng Lai Lu, Jin Lei Liu, Dian Peng Li, Jian Zhang Wu, Yue Yuan Chen, Yong Xin Wen
• Keywords: D101 Resin, Enzyme-Assisted Extraction, EsculeosideA, HPLC-ELSD, Lycopersicon esculentum MILL

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