Carbon Nanotubes for the Development of Glucose Biosensors Based on Gold Electrodeposition

Abstract:

^{Superscript text}_{Subscript text}Our study is to develop a general design of biosensors based on vertically aligned Carbon Nanotube (CNT) arrays. Glucose biosensor is selected as the model system to verify the design of biosensors. In the preliminary design, glucose oxidase (GOx) is attached to the walls of the porous alumina membrane by adsorption. Porous highly ordered anodized aluminum oxide (AAO) are used as templates. Deposited gold on both sides of template surfaces serve as a contact and prevent non-specific adhesion of GOx on the surface. In order to find out optimized thickness of gold coating, the redox reaction in([Fe(CN)₆]^3-/[Fe(CN)6]4-system is monitored by CV. Subsequently, enzymatic redox reaction in glucose solutions is also attempted by CV. We expect protein layers with GOx from a conductive network. To take advantage of the attractive properties of CNTs, the design of enzyme electrodes is modified by attaching CNT onto the sidewalls of AAO template nanopores and then immobilizing GOx to the sidewalls and tips of CNTs. Cobalt is used as a catalyst to fabricate CNTs. As a result, MWCNTs are fabricated inside the AAO templates by CCVD.

• Periodical: Advanced Materials Research (Volumes 403-408)
• Pages: 1157-1162
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.403-408.1157
• Online since: November 2011
• Authors: Firouzeh Karimi Moghadam, Meysam Hamzehlooei
• Keywords: Carbon Nanotube (CN), Glucose Biosensors, Gold Electrodiposition

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DOI: 10.1016/j.diamond.2008.04.016

[12] Hou, S.; Wang, J.; Martin, C. R. Nano Lett. 2005, 5, 231-4. Fig 1. Schematic diagrams of CNT-based electrode fabrication (a) and GOx immobilization (b). Fig 2. Top-view (a) and cross-section (b) SEM images of AAO template after two-step anodization. Fig 3 . Top-view SEM image of AAO template after pore widening. Fig 4 . Cyclic voltammogram of homemade AAO template with different thickness of gold coating. Fig 5. Top-view SEM image of empty homemade AAO template with 50nm thickness gold coating. Fig 6. Top-view (a) and cross-section (b) SEM images of AAO template after cobalt deposition. Fig 7. (a) Top-view and (b) cross-section SEM images of AAO templates after 1h CNT growth. Fig 8. High resolution top-view SEM images of AAO templates with CNTs inside nanopores after 5h etching. Fig 9. TEM images of MWCNT with an open tip (b, c) and bubble texture (a). Fig 10. Top-view SEM images of AAO template after 2h (a), 4h (b) and 10h (c) CNT growth Fig 11. Cross-section SEM image of homemade AAO template for 4 h CNT growth.