Temperature Characteristic of Immobilization Proteins on Pt Surface Based on Silicon Substrate

Abstract:

This paper investigated a method that proteins were immobilized on platinum surface based on silicon substrate according to the concept self-assembled monolayers. At first, we produced actived carboxyl groups through surface modification of platinum surface on silicon substrate in reaction solution, and then utilized condensation reaction between proteins and carboxyl groups to immobilize proteins (cattle IgG). Secondly, we showed SEM photographs of surface morphologies of immobilization cattle IgG on silicon wafer. EDS energy spectrum microanalysis of cattle IgG immobilization on silicon wafer was also obtained by means of SEM. By contrast,it was evident that proteins (cattle IgG) can be immobilized effectively on the platinum surface on silicon using the experimental methods. Finally, there were tests of I-V characteristic and I-T characteristic of immobilized cattle IgG which demonstrated its temperature coefficient is .

• Periodical: Advanced Materials Research (Volume 413)
• Pages: 138-143
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.413.138
• Online since: December 2011
• Authors: Xin Ju, Dian Zhong Wen, Ping Wang, Yu Jie Ding, Chang Hao Feng
• Keywords: Mmobilization of Proteins, Self-Assembled Monolayer (SAM), Temperature Characteristic

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