Performance of Denitrifying Phosphorous Removal Process Employing Nitrite as Electron Acceptor and the Characterization of Dominant Functional Populations

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Abstract:

A sequencing batch reactors (SBR) was adopted to investigate the denitrifying phosphorus removal efficiency employing nitrite as electron acceptor under anaerobic/anoxic condition. The experimental results showed that high nitrogen and phosphorus removal efficiency could be obtained under the following conditions: nitrite concentration of 30~40 mg/L, COD concentration of 400 mg/L, pH 8.0±0.2 in anaerobic stage and pH 7.2±0.2 in anoxic stage, sludge retention time (SRT) of 22 days. When the reactor performed steadily, a dominant functional strain was screened from the activated sludge, which has high nitrite and phosphorus removal efficiency. Batch tests results showed that the removal degree of nitrite and phosphorus could reach 99.18% and 84.94% respectively when their concentrations were 20mg/L and 10mg/L. according to the morphology and physio-biochemical characteristics, and the results of 16S rDNA sequencing, it is determined that the strain belongs to the Genus of Sphingobacterium. The experimental results achieved in this study might offer guidance to the development of shortcut denitrifying phosphorus removal process.

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Advanced Materials Research (Volumes 455-456)

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1019-1024

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January 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] Ma J,Peng Y Z,Wang S Y. Denitrifying phosphorus removal in a step-feed CAST with alternating anoxic-oxic operational strategy. Journal of Environmental Sciences. 2009, 21(9): 1169-1174.

DOI: 10.1016/s1001-0742(08)62398-0

Google Scholar

[2] Yong Z P. Chang Y W. Ran D Wang. Denitrifying phosphorus removal with nitrite by a real-time step feed sequencing batch reactor. Journal of Chemical Technology & Biotechnology. Article first published online: 23 DEC (2010).

DOI: 10.1002/jctb.2548

Google Scholar

[3] Luo G Y, Ji F Y, Luo N. Denitrifying phosphorus removal process using nitrate as electron acceptor. Environmental Engineering, 2004, 22 (1): 32-35.

Google Scholar

[4] Lin Y, Yang Y Z. Influence of nitrate concentration and anaerobic/ aerobic time on the denitrifying phosphorus removal process. Water & Wastewater, 2003, 29 (4): 36-41.

Google Scholar

[5] Luo G Y, Luo N, Ji F Y. An innovative process of denitrifying phosphorus removal with di-sludge system. China Water & Wastewater, 2002, 18 (9): 4-7.

Google Scholar

[6] Li Z Y, Peng Y Z. Influence of nitrate concentration and its adding way on the denitrifying phosphorus removal process. Environmental Pollution and Control, 2003, 25 (6): 323-325.

Google Scholar

[7] Dae Sung Lee, Che Ok Jeon and Jong Moon Park. Biological nitrogen removal with enhanced phosphate uptake in A Sequencing Batch reactor using single system. Water Research. 2001, 35(16): 3968-3976.

DOI: 10.1016/s0043-1354(01)00132-4

Google Scholar

[8] Hu, J.Y., S.L. Ong, W.J. Ng, F. Lu and X.J. Fan, A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptors. Water Research. 37, 14 (2003): 3463-3471.

DOI: 10.1016/s0043-1354(03)00205-7

Google Scholar

[9] Yang C H, Sun L P, Li X M, et al. Preliminary study of nitrate and nitrite as finalel ectron acceptor of biological phosphorous removal. Journal of TianjinInstitute of Urban Construction. 2005, 11(2): 102-106.

Google Scholar

[10] Dong X ZH, Cai M Y. Ordinary bacteria system identified manual. Peking: Science publisher, (2001).

Google Scholar

[11] Meinhold J, Arnold E and Isaacs S. Effect of nitrte or anoxic phosphate uptake in biological phosphorus removal activatrd sludge. Water Research. 1999, 33(8): 1871-1883.

DOI: 10.1016/s0043-1354(98)00411-4

Google Scholar

[12] Wachtmeister A, Kuba T, Van Loosdrecht M C M, et al. A sludge characterization assay for aerobic and denitrifying phosphorus removing sludge. Water Research. 1997, 31(3): 471–478.

DOI: 10.1016/s0043-1354(96)00281-3

Google Scholar