FTIR/ATR Spectroscopy with Stability for Determination of HGB in Human Whole Blood

Abstract:

Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) combined with partial least-squares (PLS) and Savitzky-Golay (SG) smoothing were successfully applied for rapid no-reagent determination of hemoglobin (HGB) in human whole blood. The optimal SG smoothing parameters OD, DP, NSP and PLS factor were 1, 2, 49 and 14, respectively. The modeling effect M-SEPAve, M-RP,Ave, M-SEPStd, and M-RP,Std were 6.73g/L, 0.850, 0.306g/L, and 0.015, respectively. The validation effect V-SEP and V-RP were 6.83g/L and 0.864, respectively. The method provided a reliable model with stability for clinic application of FTIR/ATR.

• Periodical: Advanced Materials Research (Volumes 468-471)
• Pages: 1663-1666
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.468-471.1663
• Online since: February 2012
• Authors: Tao Pan, Wen Jie Huang, Hao Yin
• Keywords: FTIR/ATR Spectroscopy, HGB, Human Whole Blood, PLS, SG Smoothing, Stability

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