The Effects of adhE Deletion on the Metabolism for D-Lactic Acid Production by the Escherichia coli JH11

Abstract:

Escherichia coli W produces a mixture of organic acids during fermentation in mineral salts medium using glucose as the sole carbon source. Among these products, D-lactate, acetate, succinate, and ethanol are the majors, with formate as a minor. In order to evaluate the effect of adhE mutation on the metabolism for D-lactic acid production by E. coli W, an adhE deletion mutant JH11 was constructed using the RED recombination system and the flipase recognition target （FRT） site-specific recombinant technology. Compared to the parent strain, JH11 produced significantly higher concentration of D-lactate due to the increased NADH availability, with slightly changed acetate (increased), and succinate (decreased), in fermentations using mineral salts medium containing glucose as the carbon source and calcium carbonate as the neutralizer.

• Periodical: Advanced Materials Research (Volumes 476-478)
• Pages: 2051-2054
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.476-478.2051
• Online since: February 2012
• Authors: Jin Fang Zhao, Li Yuan Xu, Yong Ze Wang, Jin Hua Wang, Sheng De Zhou
• Keywords: Alcohol Dehydrogenase, D-Lactic Acid, Escherichia coli, Gene Knockout

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