A Study of DNA Combing Speed in Fabricating Nanochannel ElectroPoration (NEP) Chips

Samuel I. En Lin

doi:10.4028/www.scientific.net/AMR.486.18

Paper Titles

Preface and Committees

Effect of Thermal Treatment Temperature of Electrospun PET Nanofiber Mat on Tensile Properties
p.3

Influence of Mo Content of Fe/Mo/Al₂O₃ on Flame Synthesis of Carbon Nanotubes
p.8

Experimental Research of Visible Light-Induced Photocatalytic Oxidation Effects of SO₂ by N-Doping Nano-TiO₂
p.12

A Study of DNA Combing Speed in Fabricating Nanochannel ElectroPoration (NEP) Chips
p.18

A ZnO Driven Silicon Cantilever for Nanoscale Actuation
p.23

Effect of Pd Nanoparticle on the Thermal Degradation Kinetics of Nylon 6/Pd Nanocomposite Prepared by a Dry Process
p.27

Effect of Injection Molding Process on Electrical Conductivity and Mechanical Property of Nanoparticle Filled Polymer Composites
p.34

Room-Temperature ZnO Nanoparticle Ethanol Gas Sensors under UV Illumination
p.39

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 486A Study of DNA Combing Speed in Fabricating...

A Study of DNA Combing Speed in Fabricating Nanochannel ElectroPoration (NEP) Chips

Abstract:

Electroporation through nanochannels has potential as a useful tool for cell transfection. This potential is due to: the low voltage required; the centralized distribution of the potential penetration; the fact that this method causes no harm to the cell membrane, and; the even expression pattern of the target gene after electroporation. Additionally, the stable production process and improved yield rate can reduce the cost of producing the nanochannels and thus make the commercialization of this technique more feasible. This study aims to investigate the relationship between the speed of DNA stretching and the yield rate of nanochannels. We found that when the length of nanochannels is 2 µm, the yield rate can exceed 90% at a stretching speed of 2.3 mm/s . With a similarly high yield rate, longer nanochannels (3 µm) displayed a wider range of stretching speed. We have determined that the stretching speed can influence the adhesion of DNA and the subsequent fabrication of nanochannels. Therefore, this speed must be appropriately controlled.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volume 486)

Pages:

18-22

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.486.18

Citation:

Cite this paper

Online since:

March 2012

Authors:

Samuel I. En Lin

Keywords:

Biochip, DNA, Electroporation, Nanochannel

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Y.W. Won, K.S. Lim, Y.H. Kim, Intracellular organelle-targeted non-viral gene delivery systems, Journal of Controlled Release 152 (2011) 99-109.

DOI: 10.1016/j.jconrel.2011.01.013

Google Scholar

[2] S.C. Yang, K.S. Huang, H.Y. Chen, Y.C. Lin, Determination of optimum gene transfection conditions using the Taguchi method for an electroporation microchip, Sensors and Actuators B132(2008)551-557.

DOI: 10.1016/j.snb.2007.11.037

Google Scholar

[3] T. Geng,Y. h. Zhan, H.Y. Wang, S.R. Witting, K.G. Cornetta, C. Lu, Flow-through electroporation based on constant voltage for large-volume transfection of cells, Journal of Controlled Release 144 (2010) 91-100.

DOI: 10.1016/j.jconrel.2010.01.030

Google Scholar

[4] X. Wen , Hongyan He , L. James Lee, Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips, Journal of Immunological Methods 350 (2009) 97–105.

DOI: 10.1016/j.jim.2009.07.011

Google Scholar

[5] P.E. Boukany, A. Morss, W.C. Liao, B. Henslee, X. Zhang, B. Yu,X. Wang, Y. Wu, H.C. Jung,L. Li, K. Gao, X. Hu, X. Zhao, O. Hemminger,W. Lu, G.P. Lafyatis and L.J. Lee, Nano channel electroporation delivers preciseamounts of biomolecules into living cells, , Nature nanotechnology, 2011, November.

DOI: 10.1038/nnano.2011.164

Google Scholar

[6] Z.Z. Fei,X. Hu, H.W. Choi, S. Wang, D. Farson, and L.J. Lee, Micronozzle Array Enhanced Sandwich Electroporation of Embryonic Stem Cells, Analytical Chemistry, Vol. 82, No. 1, pp, 353-358, January 1, (2010).

DOI: 10.1021/ac902041h

Google Scholar