Assessment of Inocula and N-Removal Performance of Anaerobic Ammonium Oxidation (ANAMMOX) for the Treatment of Aged Landfill Leachates

Abstract:

Three kinds of seeding sludge, i.e. conventional activated sludge, anaerobic granular sludge and the nitrifying activated sludge from the nitritation reactor treating aged leachates were evaluated in batch mode to screen the optimized inoculum for the rapid start-up of ANAMMOX reactor. The feasibility of the ANAMMOX process for the treatment of aged leachates was also investigated in a modified upflow anaerobic sludge blanket (UASB, 0.05m3). The batch experiments revealed that the nitrifying activated sludge from the nitritation reactor could respectively achieve the NRR (nitrogen removal rate) of 0.0365 kg N/(m3.d) and the ARR (ammonium removal rate) of 0.013 kg N/(m3.d) on day 12, which were greatly higher than those of the other two tested sludge samples. The mixture of the aforementioned nitrifying activated sludge and anaerobic granular sludge was established as an effective inoculum for the prompt start-up of ANAMMOX reactor. The maximum total nitrogen removal rate of 0.826 kg N/(m3.d) could be obtained for the treatment of “old” leachates under NLR (nitrogen loading rate) of 1.028 kg N/(m3.d). It is concluded that the N-removal performance of ANAMMOX process is still to be improved for actual engineering application to aged landfill leachates.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 2391-2398
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.2391
• Online since: May 2012
• Authors: Yan He, Gong Ming Zhou, Min Sheng Huang, Min Tong
• Keywords: Aged Landfill Leachates, Anammox, Inocula, Start-Up

References

[1] X.Z. Li, Q.L. Zhao, Recovery of ammonium-nitrogen from landfill leachate as a multi-nutrient fertilizer, Eco.Eng. 20(2003) 171-181.

DOI: 10.1016/s0925-8574(03)00012-0

[2] M.S.M. Jetten, S.J. Horn, M.C.M. van Loosdrecht, Towards a more sustainable wastewater treatment system, Water Sci.Technol.35 (1997)171-179.

DOI: 10.2166/wst.1997.0341

[3] Y. He, G.M. Zhou Y.C. Zhao, Nitrification with high nitrite accumulation for the treatment of "Old" landfill leachates, Environ. Eng. Sci.24(2007)1084-1094.

DOI: 10.1089/ees.2006.0250

[4] N. Chamchoi, S. Nitisoravut, Anammox enrichment from different conventional sludges, Chemosphere.66(2007)2225-2232.

DOI: 10.1016/j.chemosphere.2006.09.036

[5] A. Dapena-Mora, J.L. Campos, A. Mosquera-Corral, M.S.M. Jetten, R.Mendez, Stability of the ANAMMOX process in a gas-lift reactor and a SBR, J.Biotechnol. 110(2004)159-170.

DOI: 10.1016/j.jbiotec.2004.02.005

[6] I. Tsushima,Y.Ogasawara, T. Kindaichi, H.Satoh, S.Okabe, Development of high-rate anaerobic ammonium-oxidizing (anammox) biofilm reactors.Water Res.41(2007)1623-1634.

DOI: 10.1016/j.watres.2007.01.050

[7] U.Van Dongen, Jetten M.S.M., M.C.M. van Loosdrecht, The SHARON-ANAMMOX process for treatment of ammonium rich wastewater, Water Sci. Technol.44(2001)153-160.

DOI: 10.2166/wst.2001.0037

[8] X. Dong, E.W. Tollner, Evaluation of anammox and denitrification during anaerobic digestion of poultry manure, Bioresource Technol.86(2003)139-145.

DOI: 10.1016/s0960-8524(02)00154-2

[9] K.Isaka, T. Sumino , S.Tsuneda, High nitrogen removal performance at moderately low temperature utilizing anaerobic ammonium oxidation reactions, J.Biosci.Bioeng. 103(2007)486-490.

DOI: 10.1263/jbb.103.486

[10] P.Zheng, F.M. Lin, B.L.Hu, J.S. Chen, Strart-up of anaerobic ammonia oxidation bioreactor with nitrifying activated sludge, J. Environ. Sci. 16(2004a)13-16.

[11] J.L. Wang , J.Kang, The characteristics of anaerobic ammonium oxidation (ANAMMOX) by granular sludge from an EGSB reactor, Process Biochem.40(2005)1973-1978.

DOI: 10.1016/j.procbio.2004.08.001

[12] B.K. Pathak, F.Kazama, Y.Saiki, T.Sumino, Presence and activity of anammox and denitrification process in low ammonium-fed bioreactors, Bioresource Technol. 98(2007)2201-2206.

DOI: 10.1016/j.biortech.2006.08.014

[13] American Public Health Association, APHA, Standard Methods for the Examination of Water and Wastewater, Washington, DC, USA,1998.

[14] M.Strous, J.G. Kuenen, M.S.M. Jetten, Key physiology of anaerobic ammonium oxidation, Appl. Environ.Microbiol.65(1999)3248-3250.

DOI: 10.1128/aem.65.7.3248-3250.1999

[15] M.Strous, E.van Gerven, J.G. Kuenen, M.S.M. Jetten, Effects of aerobic and microaerobic conditions on anaerobic ammonium oxidizing(Anammox)sludge, Appl. Environ.Microbiol.63(1997)2446-2448.

DOI: 10.1128/aem.63.6.2446-2448.1997

[16] A.A. Van de Graaf, P. de Bruijn, L.A. Robertson, M.S.M. Jetten, J.G. Kuenen, Autotrophic growth of anaerobic ammonium oxidizing microorganisms in a fluidized bed reactor, Microbiol.142(1996) 2187 -2196.

DOI: 10.1099/13500872-142-8-2187