Implication of Temporal Dynamics of Microbial Abundance and Nutrients to Soil Fertility under Biochar Application – Field Experiments Conducted in a Brown Soil Cultivated with Soybean, North China

Abstract:

A short-term experiment was conducted to investigate responses of microbial community composition and nutrients dynamics to biochar-amended brown soil. We examined the effect of biochar on microbial abundance by plate counting method and microbial community composition changes by DGGE, as well as effect on total and available nutrients N, P and K content. Soil pH measurement was also incorporated into our study. The overall results showed that bacterial abundance increased more than actinomycetes, but fungal abundance decreased slightly in biochar amended soil in later sampling times. In terms of microbial community composition, DGGE analysis for bacteria exhibited some specific lanes in biochar treatments. Bacterial community was more sensitive to sampling time, but fungal community was influenced greatly by biochar addition. In our study, total C content increased significantly, as biochar rate and sampling time preceded. Total N increased slightly in later sampling time, and thus C and N ratio increase was obtained. Total P and K changes were not obvious. Extractable N and pH increased. Microbial utilization was assumed to contribute to extractable P and K decrease in later sampling times. Results suggested that biochar incorporation to brown soil might bring potential benefit to soil fertility from N retention in soil. Microbial turnover may feedback P and K to soil as well in the long term. There may also be an implication of beneficial effect on disease alleviation lead by microbial community imbalance.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 384-394
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.384
• Online since: May 2012
• Authors: Da Quan Sun, Meng Jun, Wei Ming Zhang, Xue Chao Guan, Yu Wei Huang, Yu Lan, Ji Ping Gao, Wen Fu Chen
• Keywords: Biochar, DGGE, Soil Nutrient

References

[1] Demirbas, A. (2004). "Effects of temperature and particle size on bio-char yield from pyrolysis of agricultural residues." Journal of Analytical and Applied Pyrolysis 72(2): 243-248.

DOI: 10.1016/j.jaap.2004.07.003

[2] Ioannidou, O. and A. Zabaniotou (2007). "Agricultural residues as precursors for activated carbon production--A review." Renewable and Sustainable Energy Reviews 11(9): 1966-2005.

DOI: 10.1016/j.rser.2006.03.013

[3] Chan, K. Y., L. Van Zwieten, et al. (2007). "Agronomic values of green waste biochar as a soil amendment." Soil Research 45(8): 629-634.

DOI: 10.1071/sr07109

[4] Lima, I. M., A. McAloon, et al. (2008). "Activated carbon from broiler litter: Process description and cost of production." Biomass and Bioenergy 32(6): 568-572.

DOI: 10.1016/j.biombioe.2007.11.008

[5] Chan, K. Y., L. Van Zwieten, et al. (2008). "Using poultry litter biochars as soil amendments." Soil Research 46(5): 437-444.

DOI: 10.1071/sr08036

[6] Lehmann, J., Joseph, S., 2009. Biochar for environmental management: an introduction. In: Lehmann, J., Joseph, S. (Eds.), Biochar for Environmental Management: Science and Technology. Earthscan, London, pp.1-12.

DOI: 10.4324/9780203762264

[7] Laird, D. A., P. Fleming, et al. (2010). "Impact of biochar amendments on the quality of a typical Midwestern agricultural soil." Geoderma 158(3-4): 443-449.

DOI: 10.1016/j.geoderma.2010.05.013

[8] Wilhelm, W.W., Doran, J.W., Power, J.F., 1986. Corn and soybean yield response to crop residue management under no-tillage production systems. Agronomy Journal 78, 184–189.

DOI: 10.2134/agronj1986.00021962007800010036x

[9] Anderson, C. R., L. M. Condron, et al. (2011). "Biochar induced soil microbial community change: Implications for biogeochemical cycling of carbon, nitrogen and phosphorus." Pedobiologia 54(5-6): 309-320.

DOI: 10.1016/j.pedobi.2011.07.005

[10] Kim, J.-S., Sparovek, S., Longo, R.M., De Melo, W.J., Crowley, D., 2007. Bacterial diversity of terra preta and pristine forest soil from the Western Amazon. Soil Biology and Biochemistry 39, 648-690.

DOI: 10.1016/j.soilbio.2006.08.010

[11] O'Neill, B., Grossman, J., Tsai, M.T., Gomes, J.E., Lehmann, J., Peterson, J., Neves, E., Thies, J.E., 2009. Bacterial community composition in Brazilian Anthrosols and adjacent soils characterized using culturing and molecular identification. Microbial Ecology 58, 23-35.

DOI: 10.1007/s00248-009-9515-y

[12] Liang, B., Lehmann, J., Sohi, S.P., Thies, J.E., O'Neill, B., Trujillo, L., Gaunt, J., Solomon, D., Grossman, J., Neves, E.G., Luizão, F.J., 2010. Black carbon affects the cycling of non-black carbon in soil. Organic Geochemistry 41, 206-213.

DOI: 10.1016/j.orggeochem.2009.09.007

[13] Grossman, J.M., O'Neill, B.E., Tsai, S.M., Liang, B., Neves, E., Lehmann, J., Thies, J.E., 2010. Amazonian anthrosols support similar microbial communities that differ distinctly from those extant in adjacent, unmodified soils of the same mineralogy. Microbial Ecology 60, 192-205.

DOI: 10.1007/s00248-010-9689-3

[14] Jin, H., 2010. Characterization of microbial life colonizing biochar and biochar amended soils. PhD Dissertation, Cornell University, Ithaca, NY.

[15] Pietikäinen, J., Kiikkilä, O., Fritze, H., 2000. Charcoal as a habitat for microbes and its effects on the microbial community of the underlying humus. Oikos 89, 231-242.

DOI: 10.1034/j.1600-0706.2000.890203.x

[16] Steiner, C., Teixeira, W.G., Lehmann, J., Zech, W., 2004. Microbial response to charcoal amendments of highly weathered soils and Amazonian Dark Earths in Central Amazonia e preliminary results. In: Glaser, B., Woods, W.I. (Eds.), Amazonian Dark Earths: Explorations in Time and Space. Springer, Berlin, Germany, pp.195-212.

DOI: 10.1007/978-3-662-05683-7_15

[17] Wardle, D.A., Nilsson, M.C., Zackrisson, O., 2008. Fire-derived charcoal causes loss of forest humus. Science 320 629-629.

DOI: 10.1126/science.1154960

[18] Kuzyakov, Y., Subbotina, I., Chen, H., Bogomolova, I., Xu, X., 2009. Black carbon decomposition and incorporation into microbial biomass estimated by 14C labeling. Soil Biology and Biochemistry 41, 210-219.

DOI: 10.1016/j.soilbio.2008.10.016

[19] Lehmann, J., M. C. Rillig, et al. (2011). "Biochar effects on soil biota – A review." Soil Biology and Biochemistry 43(9): 1812-1836.

DOI: 10.1016/j.soilbio.2011.04.022

[20] Muyzer, G., De Waal, E.C., Uitterlinden, A.G., 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695-700.

DOI: 10.1128/aem.59.3.695-700.1993

[21] Gardes, M. and T. D. Bruns (1993). "ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts." Molecular Ecology 2(2): 113-118.

DOI: 10.1111/j.1365-294x.1993.tb00005.x

[22] Andrews, S.S., Karlen, D.L., Cambardella, C.A., 2004. The soil management assessment framework: a quantitative soil quality evaluation method. Soil Science Society of America Journal 68, 1945–1962.

DOI: 10.2136/sssaj2004.1945

[23] Rondon, M.A., Molina, D., Hurtado, M., Ramirez, J., Lehmann, J., Major, J., Amezquita, E., 2006. Enhancing the productivity of crops and grasses while reducing greenhouse gas emissions through biochar amendments to unfertile tropical soils. In: Proceedings of the 18th World Congress of Soil Science, July 9–15, 2006, Philadelphia, PA, USA, p.138–168.

[24] Gaskin JW, Steiner C, Harris K, Das KC, Bibens B (2008) Effect of low-temperature pyrolysis conditions on biochar for agricultural use. T Asabe 51:2061–(2069)

DOI: 10.13031/2013.25409

[25] Chapin III F S, Barsdate R J and Barel D 1978 Phosphorus cycling in Alaskan coastal tundra: a hypothesis for the regulation of nutrient cycling. Oikos 31, 189–199.

DOI: 10.2307/3543562

[26] Walbridge MR 1991 Phosphorus availability in acid organic soils of the lower North Carolina coastal plain. Ecology 72, 2083–2100.

DOI: 10.2307/1941561

[27] Leinweber, P., Kruse, J., Walley, F.L., Gillespie, A., Eckardt, K.-U., Blyth, R., Regier, T., 2007. Nitrogen K-edge XANES - an overview of reference compounds used to identify 'unknown' organic nitrogen in environmental samples. Journal of Synchrotron Radiation 14, 500-511.

DOI: 10.1107/s0909049507042513

[28] Lehmann, J., da Silva Jr., J.P., Steiner, C., Nehls, T., Zech, W., Glaser, B., 2003. Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil 249, 343-357.

DOI: 10.1023/a:1022833116184

[29] Rondon, M.A., Lehmann, J., Ramirez, J., Hurtado, M., 2007. Biological nitrogen fixation by common beans (Phaseolus vulgaris L.) increases with biochar additions. Biol. Fertil. Soils 43, 699–708.

DOI: 10.1007/s00374-006-0152-z

[30] Yamato, M., Okimori, Y., Wibowo, I.F., Anshori, S., Ogawa, M., 2006. Effects of the application of charred bark of Acacia mangium on the yield of maize, cowpea and peanut, and soil chemical properties in South Sumatra, Indonesia. Soil Science and Plant Nutrition 52, 489-495.

DOI: 10.1111/j.1747-0765.2006.00065.x

[31] Patric Lehmann, J., da Silva Jr., J.P., Steiner, C., Nehls, T., Zech, W., Glaser, B., 2003. Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil 249, 343-357.

DOI: 10.1023/a:1022833116184

[32] kWH and Khalid R A 1974 Phosphate release and sorption by soils and sediments: effect of aerobic and anaerobic conditions. Science 186, 53–55.

DOI: 10.1126/science.186.4158.53

[33] Richardson C J 1985 Mechanisms controlling phosphorus retention capacity in freshwater wetlands. Science 228, 1424–1427.

DOI: 10.1126/science.228.4706.1424

[34] Rousk, J., Bååth, E., Brookes, P.C., Lauber, C.L., Lozupone, C., Caporaso, J.G., Knight, R., Fierer, N., 2010. Soil bacterial and fungal communities across a pH gradient in an arable soil. The ISME 4, 134-151.

DOI: 10.1038/ismej.2010.58

[35] Kara, O. and I. Bolat (2009). "Short-term effects of wildfire on microbial biomass and abundance in black pine plantation soils in Turkey." Ecological Indicators 9(6): 1151-1155.

DOI: 10.1016/j.ecolind.2009.01.002

[36] Ibekwe, A.M., Kennedy, A.C., Frohne, P.S., Papienik, S.K., Yang, C.-H., Crowley, D.E., 2002. Microbial diversity along a transect of agronomic zones. FEMS Microbial. Ecol. 39, 183–191.

DOI: 10.1111/j.1574-6941.2002.tb00921.x

[37] Horner-Devine, M.C., Carney, K.M., Bohannan, B.J.M., 2004. An ecological perspective on bacterial biodiversity. Proc. R. Soc. Lond. 271, 113–122.

DOI: 10.1098/rspb.2003.2549

[38] Yao, S., Merwin, I.A., Bird, G.W., Abawi, G.S., Thies, J.E., 2005. Orchard floor management practices that maintain vegetative or biomass groundcover stimulate soil microbial activity and alter soil microbial community composition. Plant Soil 271, 377–389.

DOI: 10.1007/s11104-004-3610-0