Isolation and Identification of Nitrobacteria Adapted to Low Temperature

Yun Hao; Xiu Guang Jiang; Qing Tian; Ai Yin Chen; Bao Ling Ma

doi:10.4028/www.scientific.net/AMR.518-523.406

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Isolation and Identification of Nitrobacteria...

Isolation and Identification of Nitrobacteria Adapted to Low Temperature

Abstract:

In this study, a scientific method which can be used to improve nitrification process at low temperature in the sewage treatment plant was introduced. The activated sludge samples were taken from aeration tank of the sewage treatment plant when the outside temperature was below 0°C (water temperature below 12 °C). Five kinds of nitrobacteria strains with cold-resistance and higher activity of ammonia degradation were isolated from aeration tanks. The physiological properties showed the five strains were identified into Sphingobacteriaceae、Rhodanobacter sp.、Pseudomonas sp.、Pandoraea sp. and Perlucidibaca piscinae. All of the strains could convert ammonia-nitrogen or NO2- into NO3- in the medium at 10°C. The ammonia and nitrate removal efficiency could be reached 80.9% and 80.3% respectively. Comparing to the unvaccinated one, the removal efficiency can be increased by 50%, which proved the isolated nitrobacteria could be applied to biological nitrification process of sewage treatment at low-temperature.

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Periodical:

Advanced Materials Research (Volumes 518-523)

Pages:

406-410

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.518-523.406

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Online since:

May 2012

Authors:

Yun Hao, Xiu Guang Jiang, Qing Tian, Ai Yin Chen, Bao Ling Ma

Keywords:

Bacteria, Isolation, Low-Temperature, Nitrification

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References

[1] L. G. Whyte, J. Hawari. Biodegradation of Varidole-Chain-Length Alkanes at Low Temperatures by a Psychrotrophic Rhodococcus sp. Appl. Environ. Microbiol. 1998, 64 (7): 257~258.

DOI: 10.1128/aem.64.7.2578-2584.1998

Google Scholar

[2] K Isaka, T Sumino High nitrogen removal performance at moderately low temperature utilizing anaerobic ammonium oxidation reactions. Journal of Bioscience and Bioengineering, 2007, 103 (5): 486~490.

DOI: 10.1263/jbb.103.486

Google Scholar

[3] Rosa Margesin. Low-temperature biodegradation of high amounts of phenol by Rhodoccus spp. and basidiomycetous yeasts. Research in Microbiology 20 05, 15 6 (1): 68~75.

DOI: 10.1016/j.resmic.2004.08.002

Google Scholar

[4] Soil Microorganism Research Association Compilation. Experimental Method of Soil Microorganism [M]．Beijing：China Social Sciences Publishing House,1983．

Google Scholar

[5] Małgorzata Komorowska-Kaufman Factors affecting the biological nitrogen removal from wastewater Process Biochemistry. 2006, 41 (5): 1015~1021.

DOI: 10.1016/j.procbio.2005.11.001

Google Scholar

[6] Yongzhen Peng, Guibing Zhu. Biological nitrogen removal with nitrification and denitrification via nitrite pathway Applied Microbiology and Biotechnology, 2006, 73: 15~26. (In Chinese)

DOI: 10.1007/s00253-006-0534-z

Google Scholar

[7] Xiuzhu Dong, Miaoying Cai. Identification Manual of Common Bacterium[M].Beijing: China Social Sciences Publishing House, 2001: 62 ~ 65. (In Chinese)

Google Scholar

[8] State Environmental Protection Administration of China《Methods for Water and Wastewater Monitoring》 editorial committee, Standard Methods for Water and Wastewater Monitoring and Analysis （4th Edition）[M]. Beijing: China Social Sciences Publishing House,1997.（In Chinese）

Google Scholar