Response Surface Optimization of Deproteinization from Shrimp Shell by Pepsin

Jing Xuan Gou; Wen Bin Dong; Qiao Zeng; Yong Ding

doi:10.4028/www.scientific.net/AMR.518-523.3999

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Response Surface Optimization of Deproteinization...

Response Surface Optimization of Deproteinization from Shrimp Shell by Pepsin

Abstract:

For the purpose of establishing a method of deprotein from the shrimp shell powder that is ready to prepare chitin by pepsin, which is a environment more friendly method compare to the other traditional methods. One factor experiment was applied to study the optimal deproteinization conditions., The data of one factor experiments indicates that the proper catalytic condition might be as follows, pH1.5, temperature 35°C, enzyme dose 500U/g, and the process time 4 hours, take the cost of production into account. On the basis of these results, response surface methodology (RSM) was used to study the optimal process conditions, the data indicates that the optimal condition occurs at 700 U/g, 36.09°C, and 5 hours, and the maximum deproteinization rate approaches 8.47%.

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Advanced Materials Research (Volumes 518-523)

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3999-4003

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https://doi.org/10.4028/www.scientific.net/AMR.518-523.3999

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May 2012

Authors:

Jing Xuan Gou, Wen Bin Dong, Qiao Zeng, Yong Ding

Keywords:

Chitin, Pepsin, Response Surface Methodology (RSM), Shrimp Shell

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