Antifungi Efficacy of the Compound Preparation of Anemarrhena asphodeloides Bunge on the Drug Resistant Strain of Candida Albicans

Abstract:

To investigate the efficacy of the compound preparation named KZY-2 which include the effective group formula of anemarrhena asphodeloides bunge and rhizoma coptidis on the fluconazole and ifraconazo resistant strains of Candida albicans respectively in vitro. The fluconazole and ifraconazo resistant strains of Candida albicans were sieved by drug sensitive scrip test and the diameter of inhibition zone was measured adopting the method of agar plate diffusion, reference to the M27-A recommended by the United States National Laboratory Standards Committee (NCCLS), the minimal inhibitive concentration(MIC,mg/m1) of KZY-2 against the the standard strain(ATCC32354) and the fluconazole and ifraconazo resistant strains of candida albicans was measured respectively. Results showed that the 12 fluconazole and ifraconazo resistant strains which named RC1 to RC12 were sieved from the 20 strains Candida albicans isolated from clinic, the diameters of inhibited zone of KZY-2 against ATCC32354 was 32mm and against RC1～RC12 was between 12mm and 28mm, the MIC of KZY-2 against ATCC32354 was 312.5μg/m1 and against RC1～RC12 was between 625μg /m1 and 5000μg /m1. These results indicate that KZY-2 has good antifungi efficacy on the standard strain and the fluconazole and ifraconazo resistant strains of Candida albicans in vitro.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 5569-5572
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.5569
• Online since: May 2012
• Authors: Lian Lan Ma, Zhi Chun Liu, Wen Ping Zhang, Hua Yan Chen, Xiao Yun Wu
• Keywords: Anemarrhena Asphodeloides Bunge, Antibacterial Action, Candida Albicans, Drug Resistant, Fluconazole

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