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Plackett-Burman Design for Screening Culture Conditions in Cellulase Production by Penicillium decumbens and Enzyme Characterization

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Abstract:

Plackett-Burman design was employed for screening culture conditions for cellulase production by Penicillium decumbens in submerged fermentation. The results showed that wheat bran was the most significant factor influencing Filter Paper Activity (FPA) of the cellulase, followed by cellulose microcrystalline and initial pH, which could be further optimized for improving the cellulase activity. The effects of pH and temperature on FPA assay were investigated, and optimal FPA could be obtained at pH 4.5 and 60 °C. The stabilities of endo-glucanase (EG), exo-glucanase (CBH) and β-glucosidase (BG) were investigated and compared with that of FPA under different pH and temperature. The results indicated that CBH and FPA were more sensitive to pH and temperature than EG and BG and the stability of CBH was very similar to that of FPA under the conditions.

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Periodical:

Advanced Materials Research (Volumes 518-523)

Pages:

5578-5585

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.518-523.5578

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Online since:

May 2012

Authors:

Nan Ban, Yu Jie Zhou, Yan Ping Ye, Lin Mei Dai, Alatangaole Damirn, Jian An Zhang, De Hua Liu

Keywords:

Cellulase, Penicillium Decumbens, Plackett-Burman Design (PBD), Stability

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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