Selective Determination of Microcystin-LR Based on Proteasomal Trypsin-Like Activity Inhibition

Abstract:

A sensitive and selective method was developed for the determination of microcystin-LR (MC-LR) based on proteasomal trypsin-like activity inhibition. Under the optimized conditions, the detection limit for MC-LR was 0.18μg/L, The linearity range was 0.2-2 μg/L，and the average recovery for MC-LR in tap water was 128%. Microcystin-YR (MC-YR) and Microcystin-RR (MC-RR) have no inhibiting effect on the proteasomal trypsin-like activity in the concentration ranged between 0.1-100 nmol/L. This method could be applied for the selective, high-throughput, and rapid determination of MC-LR in waters.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 5594-5597
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.5594
• Online since: May 2012
• Authors: Xiao Shen Lu, Shi Feng Li, Li Zhang, Xin Yu Guo, Guo Qing Shi
• Keywords: Determination, Inhibition, Microcystin, Proteasome, Trypsin-Like Activity

References

[1] Dawson R M. The toxicology of microcystins.[J]. Toxicon. 1998, 36(7): 953-962.

[2] Zhang D, Xie P, Liu Y, et al. Transfer, distribution and bioaccumulation of microcystins in the aquatic food web in Lake Taihu, China, with potential risks to human health.[J]. Sci Total Environ. 2009, 407(7): 2191-2199.

DOI: 10.1016/j.scitotenv.2008.12.039

[3] Feurstein D, Kleinteich J, Heussner A H, et al. Investigation of Microcystin Congener Dependent Uptake Into Primary Murine Neurons[J]. Environ Health Perspect. 2010.

DOI: 10.1289/ehp.0901289

[4] Trojanowicz M. Chromatographic and capillary electrophoretic determination of microcystins[J]. Sci. 2010, 33(3): 359-371.

DOI: 10.1002/jssc.200900708

[5] Moreno I M, Molina R, Jos A, et al. Determination of microcystins in fish by solvent extraction and liquid chromatography[J]. J Chromatogr A. 2005, 1080(2): 199-203.

DOI: 10.1016/j.chroma.2005.05.029

[6] Sheng J W, He M, Shi H C. A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody[J]. Anal Chim Acta. 2007, 603(1): 111-118.

DOI: 10.1016/j.aca.2007.09.029

[7] Long F, Shi H C, He M, et al. Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples.[J]. Anal Chim Acta. 2009, 649(1): 123-127.

DOI: 10.1016/j.aca.2009.07.026

[8] Voges D, Zwickl P, Baumeister W. The 26S proteasome: a molecular machine designed for controlled proteolysis.[J]. Annu Rev Biochem. 1999, 68: 1015-1068.

DOI: 10.1146/annurev.biochem.68.1.1015

[9] Shibatani T, Ward W F. Sodium dodecyl sulfate (SDS) activation of the 20S proteasome in rat liver.[J]. Arch Biochem Biophys. 1995, 321(1): 160-166.

DOI: 10.1006/abbi.1995.1381

[10] Bouaicha N, Maatouk I, Vincent G, et al. A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration.[J]. Food Chem Toxicol. 2002, 40(11): 1677-1683.

DOI: 10.1016/s0278-6915(02)00103-5