Isolation and Identification of a New Strain Acidophilic Heterotrophic Bacteria from Stone Coal Drainage

Abstract:

A strain of acidophilic heterotrophic bacteria named YY-2 was isolated from acid mine drainage collected in Yiyang, Hunan, China. The gram-negative-staining cells of YY-2 were straight long rod, 1.2～2.4 μm in length and 2.3～8.8 μm in diameter; the optimum temperature for growth was 25°C, the optimum initial pH for growth was 3.5, the optimal salt concentration was 7.5%, the maximum salt concentration was 18%. 16S rDNA gene analyses reveal that YY-2 belongs to Acidiphilium.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 598-603
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.598
• Online since: May 2012
• Authors: Li Tan, Xiao Yun Zhang, Tian Liang Cao, Dong Ying Gai, Xue Da Tian
• Keywords: Acid Mine Drainage, Acidophilic Heterotrophic Bacteria, Isolation

References

[1] Ni Shijun, Li Shan, Li Zeqin, et al. Progress in the research of acid mine drainage impact and remediation[J]. Advances in Earth Science, 2008, 23(5): 501-508.

[2] BAKER B J, BANFIELD J F. Microbial communities in acid mine drainage[J]. FEMS Microbiology Ecology, 2003, 44(2): 139-152.

DOI: 10.1016/s0168-6496(03)00028-x

[3] JOHNSON D B, HALLBERG K B. The microbiology of acidic mine waters[J]. Research in Microbiology, 2003, 154(7): 466-473.

DOI: 10.1016/s0923-2508(03)00114-1

[4] Zou Li-hong, Qian Lin, Zhang Yan-fei, et al. Isolation and identification of Acidiphilium strain DY from complex sulfide mines and its bioleaching characterization[J]. The Chinese Journal of Nonferrous Metals, 2008, 18(2): 336-341.

[5] Yang Yu, Wan Min-xin, Peng Hong, et al. Isolation and characterization of bacterium for chalcopyrite bioleaching[J]. Journal of Central South University: Science and Technology, 2008, 38(4): 641-644.

[6] Johnson D B, Halberg K B. The microbiology of acidic mine waters[J]. Research in Microbiology, 2003, 154(7): 466-473.

DOI: 10.1016/s0923-2508(03)00114-1

[7] Wu Xue-ling, Zhu Ruo-lin, Ding Jian-nan, et al. Isolation, Characterization of heterotrophic acidothermophile TC-2 and its influence on bioleaching of chalcopyrite[J]. Transactions of Nonferrous Metals Society of China, 2008, 18(12): 2259-2264.

[8] JOHNSON D B, BRIDGE T A M. Reduction of ferric iron by acidophilic heterotrophic bacteria: Evidence for constitutive and inducible enzyme systems in Acidiphilium sp.[J]. Journal of Applied Microbiology, 2002, 92:315-321.

DOI: 10.1046/j.1365-2672.2002.01535.x

[9] MANGOLD S, HARNEIT, ROHWERDER T, CLAU G S, SANDW. Novel combination of atomic force microscopy and epifluorescence microscopy for visualization of leaching bacteria on pyrite[J]. Applied and Environmental Microbiology, 2008, 74(2): 410-415.

DOI: 10.1128/aem.01812-07

[10] ZHOU Ji-kui, NIU Yin-jian. Advance in research of biological metallurgy of suifide ore[J]. Metal Mine, 2005, 346: 24-30.

[11] LIAO Meng-xia, DENG Tian-long. Biohydrometallurgical progresses on refractory sulfidesⅡ[J]. Chinese Journal of Rare Metals, 2004, 28(5): 921-935.

[12] OLSON G J, BRIERLEY J A, BRIERLEY C L. Bioleaching review part B: Progress in bioleaching: applications of microbial processes by the minerals industries[J]. Applied Microbiology and Biotechnology, 2003, 63: 249-257.

DOI: 10.1007/s00253-003-1404-6

[13] Zhang Cheng-gui, Xia Jin-lan, Qiu Guan-zhou, et al. Progress in research on Fe2+ oxidation system of Acidithiobacillus ferrooxidans[J]. Transactions of Nonferrous Metals Society of China, 2006, 16(7): 1239-1249.

[14] JOHNSON D B. Selective solid media for isolating and enumerating acidophilic bacteria[J]. Journal of Microbiological Methods, 1995, 23(2): 205-218.

DOI: 10.1016/0167-7012(95)00015-d

[15] GONZALEZ-TORIL E, LLOBET-BROSSA E, CASAMAYOR E O, AMANN R, AMILS R. Microbial ecology of an extreme acidic environment, the Tino River[J]. Applied and Environmental Microbiology, 2003, 69(8): 4853-4865.

DOI: 10.1128/aem.69.8.4853-4865.2003