DBD Plasma Induced Mutation in Phosphate-Solubilizing Bacteria Acinetobacter CalcoaceticusYC-5a

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Abstract:

An efficient agar plate mutagenesis and screening technique for improving mutation frequency was established by using dielectric barrier discharge (DBD) plasma. And very few studies have reported strains of A. calcoaceticus as phosphate solubilizing plant-associated bacteria. In this study, A.calcoaceticus showed a good ability in phosphate solubilizing. DBD plasma was conducted to A. calcoaceticus mutation for improving the phosphate-soubilizing activity. The results showed that the phosphate-soubilizing activity of mutants increased compared with original strain, and the phosphate-soubilizing activity of the best mutants up to 584.3mg/L, is 1.23-fold of original strain. It demonstrated that DBD plasma treatment has a high-efficient quality, and it will be a useful method of mutation.

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Advanced Materials Research (Volumes 535-537)

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2385-2389

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June 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] M. Del Campillo, S. Van Der Zee, and J. Torrent: Eur. J. Soil Sci. 50 (1999), pp.391-399.

Google Scholar

[2] S.A. Wakelin, R.A. Warren, P.R. Harvey, and M.H. Ryder: Biol. Fertil. Soils 40 (2004), pp.36-43.

Google Scholar

[3] P. Delvasto, A. Valverde, A. Ballester, J. Igual, J. Munoz, F. Gonzalez, M. Blazquez:Soil Biology and Biochemistry 38 (2006), pp.2645-2654.

DOI: 10.1016/j.soilbio.2006.03.020

Google Scholar

[4] H. Rodraguez, and R. Fraga:Biotechnology Advances 17 (1999), pp.319-339.

Google Scholar

[5] A. Poiata, I. Motrescu, A. Nastuta, D. Creanga, and G. Popa:Journal of Electrostatics 68 (2010), pp.128-131.

DOI: 10.1016/j.elstat.2009.11.008

Google Scholar

[6] K. Kostov, V. Rocha, C. Koga-Ito, B. Matos, M. Algatti, R. Honda, M. Kayama, and R. Mota: Surface and Coatings Technology 204 (2010), pp.2954-2959.

DOI: 10.1016/j.surfcoat.2010.01.052

Google Scholar

[7] C. Nautiyal: FEMS Microbiol. Lett. 170 (1999), pp.265-270.

Google Scholar

[8] J. Murphy, and J. Riley:Anal. Chim. Acta 27 (1962), pp.31-36.

Google Scholar

[9] H. Katznelson, E. Peterson, and J. Rouatt:Canadian Journal of Botany 40 (1962), pp.1181-1186.

Google Scholar

[10] H. Hwangbo, R.D. Park, Y.W. Kim, Y.S. Rim, K.H. Park, T.H. Kim, J.S. Suh, and K.Y. Kim:Curr. Microbiol. 47 (2003), pp.87-92.

Google Scholar

[11] P. Illmer, and F. Schinner:Soil biology and Biochemistry 27 (1995), pp.257-263.

Google Scholar

[12] R. Pecina, G. Bonn, E. Burtscher, and O. Bobleter:J. Chromatogr. A 287 (1984), pp.245-258.

Google Scholar

[13] P. Illmer, and F. Schinner:Soil biology and Biochemistry 24 (1992), pp.389-395.

Google Scholar