Oriented Immobilization of Penicillin G Acylase on the Epoxydized Magnetic Hydroxyl Particles by Phenyl Acetic Acid

Abstract:

Magnetic hydroxyl microspheres were prepared by suspension polymerization and activated by epoxy chloropropane. The magnetic particles were characterized in terms of chemical composition, particle size and electrophoretic mobility. These epoxy-activated magnetic particles were assessed as a new carries for immobilized penicillin G acylase (PGA) by covalent coupling. The oriented PGA immobilization was achieved by employing the interaction electrostatic repulsion between PGA and magnetic supports through adding the phenyl acetic acid (PAA), which resulted in a 1.194-fold increase in the enzyme activity yield as compared to that of untreated PGA. No activity of immobilized PGA was lost after 20 cycles and about 94.28% enzyme activity was retained at the end of the 80th cycle in the batch reaction system.

• Periodical: Advanced Materials Research (Volumes 550-553)
• Pages: 1345-1351
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.550-553.1345
• Online since: July 2012
• Authors: Wei Wang, Zhong Hai Li, Ji Lie Li, Yao Hui Wu
• Keywords: Immobilized Penicillin G Acylase, Magnetic Hydroxyl Microsphere, Oriented Immobilization, PAA

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